In Vitro Proliferation of Newly Bred Czech Pear Cultivars

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In Vitro Proliferation of Newly Bred Czech Pear Cultivars

Popis výsledku v původním jazyce

The objective of this study was to develop a method for rapid in vitro shoot multiplication of four newly bred Czech pear cultivars (Bohemica, Dicolor, Elektra and Erika). Six proliferation MS media containing different concentrations of cytokinins BAP (6-benzylaminopurine), TDZ (thidiazuron) or 2iP (6-(g,g-dimethylallylamino) purine) were tested. Selected pear cultivars were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Of the 80 shoot tips taken only 3 explants of Bohemica, 2 explants of Erika and 1 explant of Dicolor were visibly contaminated. The highest proliferation rate was obtained for pear cultivar Elektra that produced 4.1 shoots (longer than 10 mm) on MS medium containing 1mg/L TDZ. The lowest proliferation rate was obtained for cultivar Dicolor, which did not multiply at all on MS medium containing the lowest concentration of BAP 1 mg/L.

Název v anglickém jazyce

In Vitro Proliferation of Newly Bred Czech Pear Cultivars

Popis výsledku anglicky

The objective of this study was to develop a method for rapid in vitro shoot multiplication of four newly bred Czech pear cultivars (Bohemica, Dicolor, Elektra and Erika). Six proliferation MS media containing different concentrations of cytokinins BAP (6-benzylaminopurine), TDZ (thidiazuron) or 2iP (6-(g,g-dimethylallylamino) purine) were tested. Selected pear cultivars were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. Of the 80 shoot tips taken only 3 explants of Bohemica, 2 explants of Erika and 1 explant of Dicolor were visibly contaminated. The highest proliferation rate was obtained for pear cultivar Elektra that produced 4.1 shoots (longer than 10 mm) on MS medium containing 1mg/L TDZ. The lowest proliferation rate was obtained for cultivar Dicolor, which did not multiply at all on MS medium containing the lowest concentration of BAP 1 mg/L.

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-90-6605-612-1

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

6

Strana od-do

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Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Dresden

Datum konání akce

1. 9. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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