Preliminary Results of In Vitro Thermotherapy of Pear Cultivars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F09%3A%230000177" target="_blank" >RIV/25271121:_____/09:#0000177 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.scopus.com/inward/record.url?eid=2-s2.0-75649149476&partnerID=40&md5=ac5996f11e4672ed151669acf899f597" target="_blank" >http://www.scopus.com/inward/record.url?eid=2-s2.0-75649149476&partnerID=40&md5=ac5996f11e4672ed151669acf899f597</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preliminary Results of In Vitro Thermotherapy of Pear Cultivars

Popis výsledku v původním jazyce

Two pear cultivars Alexander Lucas and Elektra were selected for application of in vitro thermotherapy at 39 °C as a technique for virus elimination. Trees were selected and the presence of viruses was initially determined before thermotherapy by ELISA and RT-PCR . Based on the results of these tests, both pear cultivars were infected with Apple stem pitting virus (ASPV). Cultivars were successfully multiplied using in vitro cultures on MS medium with 1.5 mg/L BAP. Heat treatment (39°C) was applied to the in vitro cultures on the same MS medium with 1.5 mg/L BAP as for multiplication. Between five and 10 days of heat therapy, the green apical parts of the axis, comprising the apical meristem plus one or two leaf primordia, were dissected and transferred to a fresh multiplication MS medium. For Elektra, 100% and for Alexander Lucas, 79.2% of isolated apices survived and developed shoots. Six months after the end of heat treatment, eight clones of pear cultivar Elektra and seven clones o

Název v anglickém jazyce

Preliminary Results of In Vitro Thermotherapy of Pear Cultivars

Popis výsledku anglicky

Two pear cultivars Alexander Lucas and Elektra were selected for application of in vitro thermotherapy at 39 °C as a technique for virus elimination. Trees were selected and the presence of viruses was initially determined before thermotherapy by ELISA and RT-PCR . Based on the results of these tests, both pear cultivars were infected with Apple stem pitting virus (ASPV). Cultivars were successfully multiplied using in vitro cultures on MS medium with 1.5 mg/L BAP. Heat treatment (39°C) was applied to the in vitro cultures on the same MS medium with 1.5 mg/L BAP as for multiplication. Between five and 10 days of heat therapy, the green apical parts of the axis, comprising the apical meristem plus one or two leaf primordia, were dissected and transferred to a fresh multiplication MS medium. For Elektra, 100% and for Alexander Lucas, 79.2% of isolated apices survived and developed shoots. Six months after the end of heat treatment, eight clones of pear cultivar Elektra and seven clones o

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/1B44051" target="_blank" >1B44051: Výzkum a vývoj standardních metod ozdravení pomocí termoterapie a in vitro kultur odrůd ovocných stromů a révy vinné od virů, fytoplazem a karanténních patogenů pro systém certifikace zdravotního stavu výsadbového materiálu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-90-6605-612-1

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

7

Strana od-do

261-267

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Dresden

Datum konání akce

1. 9. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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