Elimination of Apple Stem Pitting Virus from Pear Cultivars by in Vitro Chemotherapy
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F11%3A%230000165" target="_blank" >RIV/25271121:_____/11:#0000165 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Elimination of Apple Stem Pitting Virus from Pear Cultivars by in Vitro Chemotherapy
Popis výsledku v původním jazyce
The objective of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro grown plants of infected pear cultivars Alexander Lucas, Bohemica, Elektra and Rote Williams. ASPV was proved in selected field-grown trees of these cultivars by repeated RT-PCR testing. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was added in concentration 20 mg/L after autoclaving to thesame MS medium as for multiplication. After a subculture period of 4 weeks, the apical part of the axis about 3 mm in length comprising the apical meristem plus two to three leaf primordia, which developed during chemotherapy, was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg/L BAP for regeneration. From the selected genotypes, low phytotoxicity of ribavirin in concentration 20 mg/L was observed only in the case of cultivars Alexander
Název v anglickém jazyce
Elimination of Apple Stem Pitting Virus from Pear Cultivars by in Vitro Chemotherapy
Popis výsledku anglicky
The objective of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Apple stem pitting virus (ASPV) from in vitro grown plants of infected pear cultivars Alexander Lucas, Bohemica, Elektra and Rote Williams. ASPV was proved in selected field-grown trees of these cultivars by repeated RT-PCR testing. In vitro cultures were successfully multiplied on MS medium with BAP (6-benzylaminopurine). Ribavirin was added in concentration 20 mg/L after autoclaving to thesame MS medium as for multiplication. After a subculture period of 4 weeks, the apical part of the axis about 3 mm in length comprising the apical meristem plus two to three leaf primordia, which developed during chemotherapy, was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg/L BAP for regeneration. From the selected genotypes, low phytotoxicity of ribavirin in concentration 20 mg/L was observed only in the case of cultivars Alexander
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
GF - Choroby, škůdci, plevely a ochrana rostlin
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Acta Horticulturae
ISBN
978-90-6605-584-1
ISSN
0567-7572
e-ISSN
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Počet stran výsledku
5
Strana od-do
111-115
Název nakladatele
International Society for Horticultural Science
Místo vydání
Leuven
Místo konání akce
Lisbon
Datum konání akce
22. 8. 2010
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
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