Elimination of PDV from sweet cherry cultivars by in vitro chemotherapy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000098" target="_blank" >RIV/25271121:_____/19:N0000098 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1242.4" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1242.4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1242.4" target="_blank" >10.17660/ActaHortic.2019.1242.4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Elimination of PDV from sweet cherry cultivars by in vitro chemotherapy

Popis výsledku v původním jazyce

The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Prune dwarf virus (PDV) from in vitro grown plants of infected sweet cherry cultivars ‘Tamara’ and ‘Amid’. Initial in vitro cultures were grown in Erlenmeyer flasks with 25 mL of MS medium (Murashige and Skoog, 1962), to which 1.5 mg L-1 BAP (6-benzylaminopurine) had been added. All cultures were cultivated in controlled environment chambers at 22±1°C. As soon as enough shoots were developed, individual shoots (5-10 mm in length) were excised from stock collections and transferred to treatment media with ribavirin. Ribavirin was added in concentration of 20 mg L-1 after autoclaving to the same MS medium as for multiplication. After a subculture period of 4 weeks, the apical part of the axis (about 3 mm in length comprising the apical meristem plus two-three primordial leaves) was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg L-1 BAP for regeneration. The chemotherapy technique was able to eliminate PDV in high percentage according to the cultivar, ranging between 100.0 and 96.4% for cultivars ‘Tamara’ and ‘Amid’, respectively. In the course of chemotherapy cycles, ribavirin concentrations of 20 mg L-1, ‘Amid’ plants did not display symptoms of phytotoxicity and appeared vigorous and healthy. In contrary, 35% of ‘Tamara’ in vitro plants did not survive the ribavirin treatment. The obtained results demonstrate the effectiveness of the method to eliminate PDV by a combination of in vitro cultures, chemotherapy with ribavirin and subsequent removing of apical meristematic region.

Název v anglickém jazyce

Elimination of PDV from sweet cherry cultivars by in vitro chemotherapy

Popis výsledku anglicky

The aim of the present study was to determine whether chemotherapy with ribavirin can be used to eliminate Prune dwarf virus (PDV) from in vitro grown plants of infected sweet cherry cultivars ‘Tamara’ and ‘Amid’. Initial in vitro cultures were grown in Erlenmeyer flasks with 25 mL of MS medium (Murashige and Skoog, 1962), to which 1.5 mg L-1 BAP (6-benzylaminopurine) had been added. All cultures were cultivated in controlled environment chambers at 22±1°C. As soon as enough shoots were developed, individual shoots (5-10 mm in length) were excised from stock collections and transferred to treatment media with ribavirin. Ribavirin was added in concentration of 20 mg L-1 after autoclaving to the same MS medium as for multiplication. After a subculture period of 4 weeks, the apical part of the axis (about 3 mm in length comprising the apical meristem plus two-three primordial leaves) was dissected under a laminar flow hood and transferred to a fresh multiplication MS medium with 1.5 mg L-1 BAP for regeneration. The chemotherapy technique was able to eliminate PDV in high percentage according to the cultivar, ranging between 100.0 and 96.4% for cultivars ‘Tamara’ and ‘Amid’, respectively. In the course of chemotherapy cycles, ribavirin concentrations of 20 mg L-1, ‘Amid’ plants did not display symptoms of phytotoxicity and appeared vigorous and healthy. In contrary, 35% of ‘Tamara’ in vitro plants did not survive the ribavirin treatment. The obtained results demonstrate the effectiveness of the method to eliminate PDV by a combination of in vitro cultures, chemotherapy with ribavirin and subsequent removing of apical meristematic region.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-62612-39-6

ISSN

—

e-ISSN

—

Počet stran výsledku

5

Strana od-do

31-34

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Chania, Kréta, Řecko

Datum konání akce

10. 7. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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