Evaluation of old and local apple (Malus x domestica Borkh.) varieties from genetic resources by molecular genetic SSR analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F09%3A0R000906" target="_blank" >RIV/25271121:_____/09:0R000906 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of old and local apple (Malus x domestica Borkh.) varieties from genetic resources by molecular genetic SSR analysis

Popis výsledku v původním jazyce

The worldwide apple genetic resources (1087 varieties) are maintained in the Research and Breeding Institute of Pomology Holovousy Ltd. At present, the utilisation of DNA molecular genetic methods is the best suitable method for the evaluation of individual genotypes, the elimination of duplications and characterisation of genetic relationships. In our preliminary study, we used 102 old and local apple varieties for DNA molecular genetic evaluation. SSR molecular genetic analysis of six loci was carriedout on these samples. We found a high amplified polymorphism of SSR markers among local apple varieties, as all amplified products (85) were polymorphic. A cluster analysis was used for the evaluation of genetic relationships of individual genotypes oflocal apple varieties. No duplication was found in the set of genotypes and wide molecular genetic biodiversity of local apple varieties varieties was mapped.

Název v anglickém jazyce

Evaluation of old and local apple (Malus x domestica Borkh.) varieties from genetic resources by molecular genetic SSR analysis

Popis výsledku anglicky

The worldwide apple genetic resources (1087 varieties) are maintained in the Research and Breeding Institute of Pomology Holovousy Ltd. At present, the utilisation of DNA molecular genetic methods is the best suitable method for the evaluation of individual genotypes, the elimination of duplications and characterisation of genetic relationships. In our preliminary study, we used 102 old and local apple varieties for DNA molecular genetic evaluation. SSR molecular genetic analysis of six loci was carriedout on these samples. We found a high amplified polymorphism of SSR markers among local apple varieties, as all amplified products (85) were polymorphic. A cluster analysis was used for the evaluation of genetic relationships of individual genotypes oflocal apple varieties. No duplication was found in the set of genotypes and wide molecular genetic biodiversity of local apple varieties varieties was mapped.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

<a href="/cs/project/QH72163" target="_blank" >QH72163: Hodnocení genofondu jabloní (Malus x domestica) molekulárně genetickými metodami.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agriculture

ISSN

0551-3677

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

3

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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