Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F20%3AN0000018" target="_blank" >RIV/14864347:_____/20:N0000018 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.actahort.org/books/1235/1235_9.htm" target="_blank" >https://www.actahort.org/books/1235/1235_9.htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.9" target="_blank" >10.17660/ActaHortic.2019.1235.9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Popis výsledku v původním jazyce

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of individual accessions, so that we eliminated duplications and characterized genetic relationships. Recently, there are many different molecular methods but the most reliable are SSR markers. In our work, we used PCR primer combinations for 20 SSR and 2 EST-SSR markers for analyses of 82 current, old and local sweet cherry cultivars from Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy. In total, 113 polymorphic markers were amplified which we used for hierarchical cluster analysis of genetic variability. The resulting dendrogram corresponded with genealogical and geobotanical characteristics of individual accessions which were divided into five clusters. The breeding history of several known accessions was also proved.

Název v anglickém jazyce

Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Popis výsledku anglicky

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of individual accessions, so that we eliminated duplications and characterized genetic relationships. Recently, there are many different molecular methods but the most reliable are SSR markers. In our work, we used PCR primer combinations for 20 SSR and 2 EST-SSR markers for analyses of 82 current, old and local sweet cherry cultivars from Czech genetic resources of the Research and Breeding Institute of Pomology in Holovousy. In total, 113 polymorphic markers were amplified which we used for hierarchical cluster analysis of genetic variability. The resulting dendrogram corresponded with genealogical and geobotanical characteristics of individual accessions which were divided into five clusters. The breeding history of several known accessions was also proved.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QJ1510001" target="_blank" >QJ1510001: Výzkum genofondu třešně využitím molekulárně genetických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-6261-295-2

ISSN

0567-7572

e-ISSN

2406-6168

Počet stran výsledku

8

Strana od-do

441–448

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Instanbul, Turkey

Datum konání akce

12. 8. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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