Evaluation of genetic diversity within sweet cherry accessions of Czech genetic resources by molecular SSR markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000095" target="_blank" >RIV/25271121:_____/19:N0000095 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/14864347:_____/19:N0000014

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.9" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1235.9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.9" target="_blank" >10.17660/ActaHortic.2019.1235.9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of genetic diversity within sweet cherry accessions of Czech genetic resources by molecular SSR markers

Popis výsledku v původním jazyce

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of individual accessions, so that we eliminated duplications and characterized genetic relationships. Recently, there are many different molecular methods but the most reliable are SSR markers.In our work, we used PCR primer combinations for 20 SSR and 2 EST-SSR markers for analyses of 82 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 113 polymorphic markers were amplified which we used for hierarchical cluster analysis of genetic variability. The result dendrogram corresponded with genealogical and geobotanical characteristics of individual accessions which were divided into five clusters. There was also proved the breeding history of several known accessions. This work was supported by the National Agency for Agricultural Research of the Ministry of Agriculture of CR in project QJ1510001.

Název v anglickém jazyce

Evaluation of genetic diversity within sweet cherry accessions of Czech genetic resources by molecular SSR markers

Popis výsledku anglicky

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of individual accessions, so that we eliminated duplications and characterized genetic relationships. Recently, there are many different molecular methods but the most reliable are SSR markers.In our work, we used PCR primer combinations for 20 SSR and 2 EST-SSR markers for analyses of 82 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 113 polymorphic markers were amplified which we used for hierarchical cluster analysis of genetic variability. The result dendrogram corresponded with genealogical and geobotanical characteristics of individual accessions which were divided into five clusters. There was also proved the breeding history of several known accessions. This work was supported by the National Agency for Agricultural Research of the Ministry of Agriculture of CR in project QJ1510001.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Projekt

<a href="/cs/project/QJ1510001" target="_blank" >QJ1510001: Výzkum genofondu třešně využitím molekulárně genetických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

9789462612327

ISSN

—

e-ISSN

—

Počet stran výsledku

8

Strana od-do

71-78

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Yamagata, Japonsko

Datum konání akce

5. 6. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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