Evaluation of genetic variability within sweet cherry (Prunus avium L.) genetic resources by molecular SSR markers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000019" target="_blank" >RIV/25271121:_____/19:N0000019 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/14864347:_____/19:N0000003

Výsledek na webu

<a href="https://czasopisma.up.lublin.pl/index.php/asphc/article/view/846" target="_blank" >https://czasopisma.up.lublin.pl/index.php/asphc/article/view/846</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.24326/asphc.2019.3.15" target="_blank" >10.24326/asphc.2019.3.15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of genetic variability within sweet cherry (Prunus avium L.) genetic resources by molecular SSR markers

Popis výsledku v původním jazyce

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic analyses is the best suitable method for evaluation of individual accessions, thus we eliminated duplications and characterized the genetic relationships. In our work, we used PCR primer combinations for 19 SSR and 2 EST-SSR loci for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic fragments were amplified, which we used for hierarchical cluster analysis of genetic variability. The result dendrograms were divided into three main clusters and ten subgroups. Clustering corresponded to genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.

Název v anglickém jazyce

Evaluation of genetic variability within sweet cherry (Prunus avium L.) genetic resources by molecular SSR markers

Popis výsledku anglicky

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for future breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic analyses is the best suitable method for evaluation of individual accessions, thus we eliminated duplications and characterized the genetic relationships. In our work, we used PCR primer combinations for 19 SSR and 2 EST-SSR loci for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic fragments were amplified, which we used for hierarchical cluster analysis of genetic variability. The result dendrograms were divided into three main clusters and ten subgroups. Clustering corresponded to genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1510001" target="_blank" >QJ1510001: Výzkum genofondu třešně využitím molekulárně genetických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS

ISSN

1644-0692

e-ISSN

2545-1405

Svazek periodika

18

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

9

Strana od-do

157-165

Kód UT WoS článku

000478566800014

EID výsledku v databázi Scopus

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