Evaluation of S-incompatibility locus, genetic diversity and structure of sweet cherry (Prunus avium L.) genetic resources by molecular methods and phenotypic characteristics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F20%3AN0000005" target="_blank" >RIV/14864347:_____/20:N0000005 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/25271121:_____/19:N0000022

Výsledek na webu

<a href="https://www.tandfonline.com/doi/abs/10.1080/14620316.2019.1647798?journalCode=thsb20" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/14620316.2019.1647798?journalCode=thsb20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/14620316.2019.1647798" target="_blank" >10.1080/14620316.2019.1647798</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of S-incompatibility locus, genetic diversity and structure of sweet cherry (Prunus avium L.) genetic resources by molecular methods and phenotypic characteristics

Popis výsledku v původním jazyce

Sweet cherry is a vegetatively propagated, self-incompatible, perennial plant with a high level of heterozygosity and ancient breeding history. Maintenance and conservation of genetic resources are necessary for future breeding programmes, growers and fruit production stability. The knowledge about S-allele constitution of cultivars is very important for fruit growers and breeders. DNA molecular genetic methods were used for evaluation of genetic variability and S-genotyping within sweet cherry genetic resources. Totally, 13 different S-alleles in 29 S-locus combinations for 24 individual incompatibility groups within 153 sweet cherries accessions were detected. S3 (88 cultivars), S1 (60 cultivars) and S4 (54 cultivars), followed by S2 (34 cultivars) and S6 (28 cultivars) were the most frequent S-alleles. S-alleles distribution linked to ripening time was also analysed. A total of 117 microsatellite SSR and EST-SSR polymorphic markers were used for molecular-genetic analyses. The hierarchical cluster analysis divided sweet cherry cultivars into seven clusters. Genetic relationships of individual cultivars corresponded with genealogical and geobotanical characteristics and known breeding history. Genetic population structure analyses in seven clusters (K= 7) supported hierarchical clustering results and involved ripening period, fruit colour and country of origin to genetic diversity evaluation of germplasm collection.

Název v anglickém jazyce

Evaluation of S-incompatibility locus, genetic diversity and structure of sweet cherry (Prunus avium L.) genetic resources by molecular methods and phenotypic characteristics

Popis výsledku anglicky

Sweet cherry is a vegetatively propagated, self-incompatible, perennial plant with a high level of heterozygosity and ancient breeding history. Maintenance and conservation of genetic resources are necessary for future breeding programmes, growers and fruit production stability. The knowledge about S-allele constitution of cultivars is very important for fruit growers and breeders. DNA molecular genetic methods were used for evaluation of genetic variability and S-genotyping within sweet cherry genetic resources. Totally, 13 different S-alleles in 29 S-locus combinations for 24 individual incompatibility groups within 153 sweet cherries accessions were detected. S3 (88 cultivars), S1 (60 cultivars) and S4 (54 cultivars), followed by S2 (34 cultivars) and S6 (28 cultivars) were the most frequent S-alleles. S-alleles distribution linked to ripening time was also analysed. A total of 117 microsatellite SSR and EST-SSR polymorphic markers were used for molecular-genetic analyses. The hierarchical cluster analysis divided sweet cherry cultivars into seven clusters. Genetic relationships of individual cultivars corresponded with genealogical and geobotanical characteristics and known breeding history. Genetic population structure analyses in seven clusters (K= 7) supported hierarchical clustering results and involved ripening period, fruit colour and country of origin to genetic diversity evaluation of germplasm collection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QJ1510001" target="_blank" >QJ1510001: Výzkum genofondu třešně využitím molekulárně genetických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Journal of Horticultural Science and Biotechnology

ISSN

1462-0316

e-ISSN

2380-4084

Svazek periodika

95

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

84-92

Kód UT WoS článku

000481067100001

EID výsledku v databázi Scopus

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