Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F20%3AN0000054" target="_blank" >RIV/25271121:_____/20:N0000054 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Popis výsledku v původním jazyce

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Sweet cherries are self-incompatible determined by a gametophytic self-incompatibility system (GSI), controlled by a multi-allelic S-locus. Maintenance and conservation of genetic resources are necessary for future breeding programs, growers and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of genetic variability and S-genotyping within individual accessions. In our work, we used PCR primer combinations for 20 SSR, 2 EST-SSR and 10 universal or specific S-locus markers for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic markers were amplified by SSR markers which we used for hierarchical cluster analysis of genetic variability. Clustering corresponded as with genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions. We confirmed previous S-genotyping for 72 accessions excepted Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Alfa, Huldra, Rivan, Vanda and Winkler’s Frühe. It can be due to mislabelling or mistakes in previous analysis. Totally, we detected S-locus combinations in 19 incompatibility group. The most frequent incompatibility groups were III (S3S4), II (S1S3), IV (S2S3), and VI (S3S6).

Název v anglickém jazyce

Utilization of molecular methods for evaluation of sweet cherry (Prunus avium L.) genetic resources

Popis výsledku anglicky

Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Sweet cherries are self-incompatible determined by a gametophytic self-incompatibility system (GSI), controlled by a multi-allelic S-locus. Maintenance and conservation of genetic resources are necessary for future breeding programs, growers and fruit production stability. In present, the utilization of DNA molecular genetic methods is the best suitable method for evaluation of genetic variability and S-genotyping within individual accessions. In our work, we used PCR primer combinations for 20 SSR, 2 EST-SSR and 10 universal or specific S-locus markers for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic markers were amplified by SSR markers which we used for hierarchical cluster analysis of genetic variability. Clustering corresponded as with genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions. We confirmed previous S-genotyping for 72 accessions excepted Drogans Gelbe, Hedelfinger, Erika, Meckenheimer Frühe, Alfa, Huldra, Rivan, Vanda and Winkler’s Frühe. It can be due to mislabelling or mistakes in previous analysis. Totally, we detected S-locus combinations in 19 incompatibility group. The most frequent incompatibility groups were III (S3S4), II (S1S3), IV (S2S3), and VI (S3S6).

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1510001" target="_blank" >QJ1510001: Výzkum genofondu třešně využitím molekulárně genetických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

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ISSN

0567-7572

e-ISSN

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Počet stran výsledku

8

Strana od-do

441-448

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Istanbul

Datum konání akce

12. 8. 2018

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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