In vitro establishment and proliferation of red currant cultivar 'Detvan'

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F12%3A%230000505" target="_blank" >RIV/25271121:_____/12:#0000505 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro establishment and proliferation of red currant cultivar 'Detvan'

Popis výsledku v původním jazyce

The objective of this study was to develop in vitro multiplication protocol for use with commercially important red currant cultivar 'Detvan'. Initial explants were successfully established in vitro using mercuric chloride in a concentration of 0.15% asa sterilization solution. The overall rate of contamination was 9.7% only. Most of the uncontaminated explants produced new plants in the form of rosettes with short internodes and small leaves after four weeks in culture. To evaluate the shoot multiplication two different mineral media MS and WPM containing different concentrations of growth regulators were employed. Neither of the used media promoted markedly in vitro shoot formation. On MS medium, the number of newly formed shoots in particular subcultures varied between 1.5 and 2.5. Generally, the highest multiplication rate (2.5) was obtained for 'Detvan' on MS medium with 2 mg L-1 BAP, 0.5 mg L-1 IBA and 0.1 mg L-1 GA3. On MS medium, it was possible to maintain actively growing in

Název v anglickém jazyce

In vitro establishment and proliferation of red currant cultivar 'Detvan'

Popis výsledku anglicky

The objective of this study was to develop in vitro multiplication protocol for use with commercially important red currant cultivar 'Detvan'. Initial explants were successfully established in vitro using mercuric chloride in a concentration of 0.15% asa sterilization solution. The overall rate of contamination was 9.7% only. Most of the uncontaminated explants produced new plants in the form of rosettes with short internodes and small leaves after four weeks in culture. To evaluate the shoot multiplication two different mineral media MS and WPM containing different concentrations of growth regulators were employed. Neither of the used media promoted markedly in vitro shoot formation. On MS medium, the number of newly formed shoots in particular subcultures varied between 1.5 and 2.5. Generally, the highest multiplication rate (2.5) was obtained for 'Detvan' on MS medium with 2 mg L-1 BAP, 0.5 mg L-1 IBA and 0.1 mg L-1 GA3. On MS medium, it was possible to maintain actively growing in

Druh

D - Stať ve sborníku

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

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Projekt

<a href="/cs/project/QH91224" target="_blank" >QH91224: Výzkum metod ozdravování rybízu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

9789066052086

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

4

Strana od-do

387-390

Název nakladatele

International Society for Horticultural Science (ISHS)

Místo vydání

Leuven, Belgium

Místo konání akce

Zlatibor, Serbia

Datum konání akce

22. 6. 2011

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000313283300064