Improvement of System for Testing of Pome Fruit resistance to Erwinia amylovora Using In Vitro Artificial Inoculation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F14%3A%230000585" target="_blank" >RIV/25271121:_____/14:#0000585 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Improvement of System for Testing of Pome Fruit resistance to Erwinia amylovora Using In Vitro Artificial Inoculation

Popis výsledku v původním jazyce

Fire blight is a destructive bacterial disease that presents potential thr eat to apple and pear industries throughout Europe. Specific, safe and reliable methods for identifying the pathogen Erwinia amylovora and a consistent system for cultivar resistance testing can be very important factors in the management of the disease. Therefore a screening system for testing of pome fruit resistance to E. amylovora was developed based on in vitro artificial inoculation. Selected pome fru it genotypes were successfully established in vitro using mercuric chloride in a concentration of O.I5% as a sterilization solution. MS medium according to Murashige and Skoog (I962) with addition of cytokinins 6-benzylaminopurine (BAP, 2 and 4 mg/L) or thidiazuron (TDZ, 0.5and I mg/L) proved to be suitable for in vitro proliferation. We obtained multiplication coefficients higher than 2.0 for all tested pome fruit genotypes. The application of TDZ in both concentrations induced hyperhydricity in the case of

Název v anglickém jazyce

Improvement of System for Testing of Pome Fruit resistance to Erwinia amylovora Using In Vitro Artificial Inoculation

Popis výsledku anglicky

Fire blight is a destructive bacterial disease that presents potential thr eat to apple and pear industries throughout Europe. Specific, safe and reliable methods for identifying the pathogen Erwinia amylovora and a consistent system for cultivar resistance testing can be very important factors in the management of the disease. Therefore a screening system for testing of pome fruit resistance to E. amylovora was developed based on in vitro artificial inoculation. Selected pome fru it genotypes were successfully established in vitro using mercuric chloride in a concentration of O.I5% as a sterilization solution. MS medium according to Murashige and Skoog (I962) with addition of cytokinins 6-benzylaminopurine (BAP, 2 and 4 mg/L) or thidiazuron (TDZ, 0.5and I mg/L) proved to be suitable for in vitro proliferation. We obtained multiplication coefficients higher than 2.0 for all tested pome fruit genotypes. The application of TDZ in both concentrations induced hyperhydricity in the case of

Druh

D - Stať ve sborníku

CEP obor

GF - Choroby, škůdci, plevely a ochrana rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210184" target="_blank" >QJ1210184: Využití biotechnologických metod pro zefektivnění testování rezistence jádrovin vůči patogenu bakteriální spály růžovitých (bakterie Erwinia amylovora)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

9789462610460

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

4

Strana od-do

263-266

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Zürich, Switzerland

Datum konání akce

2. 7. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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