ldentification of a cytokinin treatment for in vitro propagation of two sweet cherry cultivars in preparation of a sanitation protocol

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F17%3AN0000009" target="_blank" >RIV/25271121:_____/17:N0000009 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.22" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2017.1155.22</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2017.1155.22" target="_blank" >10.17660/ActaHortic.2017.1155.22</a>

Jazyk výsledku

angličtina

Název v původním jazyce

ldentification of a cytokinin treatment for in vitro propagation of two sweet cherry cultivars in preparation of a sanitation protocol

Popis výsledku v původním jazyce

Sweet cherry (Prunus avium L.), member of the subgenus Cerasus, is a globally important vegetatively propagated fruit crop. Unfortunately, trees grown in fields and nurseries are threatened by the Prune dwarf virus (PDV). This vir us causes malformations and a decrease in growth vigour and yield. A solution to get rid of the PDV virus in the Czech prospective sweet cherry cultivars 'Tamara' and 'Kasandra' is a part of the global sanitation programme. Tissue culture techniques are an important tool for quick multiplication of requested cultivars and offer the opportunity to improve the health status of micropropagated plants by combining in vitro culture with chemo or thermotherapy. The goal of this study was to identity factors affecting the ability of Czech sweet cherry cultivars to produce shoots under in vitro culture conditions. Six proliferation MS media containing 1, 2 and 4 mg L·l BAP (6-benzylaminopurine), O.S and 1 mg L·l TDZ (thidiazuron) or 10 mg L·t ZiP (6-(g,gdimethylallylamino) purine) were tested. Values of multiplication r ate varied between 1.2 and 4.5. From the tested cytokinins, BAP at a concentration of 4 mg L·t was found to be more effective than TDZ and ZiP for shoot multiplication. In conclusion, our experiments confirmed that in vitro propagation of selected sweet cherry cultivars can be achieved. The described procedure enabled us to multiply and maintain in vitro plants throughout the year of the two sweet cultivars for further experiments with in vitro chemotherapy.

Název v anglickém jazyce

ldentification of a cytokinin treatment for in vitro propagation of two sweet cherry cultivars in preparation of a sanitation protocol

Popis výsledku anglicky

Sweet cherry (Prunus avium L.), member of the subgenus Cerasus, is a globally important vegetatively propagated fruit crop. Unfortunately, trees grown in fields and nurseries are threatened by the Prune dwarf virus (PDV). This vir us causes malformations and a decrease in growth vigour and yield. A solution to get rid of the PDV virus in the Czech prospective sweet cherry cultivars 'Tamara' and 'Kasandra' is a part of the global sanitation programme. Tissue culture techniques are an important tool for quick multiplication of requested cultivars and offer the opportunity to improve the health status of micropropagated plants by combining in vitro culture with chemo or thermotherapy. The goal of this study was to identity factors affecting the ability of Czech sweet cherry cultivars to produce shoots under in vitro culture conditions. Six proliferation MS media containing 1, 2 and 4 mg L·l BAP (6-benzylaminopurine), O.S and 1 mg L·l TDZ (thidiazuron) or 10 mg L·t ZiP (6-(g,gdimethylallylamino) purine) were tested. Values of multiplication r ate varied between 1.2 and 4.5. From the tested cytokinins, BAP at a concentration of 4 mg L·t was found to be more effective than TDZ and ZiP for shoot multiplication. In conclusion, our experiments confirmed that in vitro propagation of selected sweet cherry cultivars can be achieved. The described procedure enabled us to multiply and maintain in vitro plants throughout the year of the two sweet cultivars for further experiments with in vitro chemotherapy.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1210175" target="_blank" >QJ1210175: Výzkum a vývoj standardních metodických postupů ozdravování ovocných dřevin a révy vinné pomocí chemoterapie in vitro kultur pro systém certifikace zdravotního stavu výsadbového materiálu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-6261-151-1

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

4

Strana od-do

161-164

Název nakladatele

ISHS

Místo vydání

Leuven 1, Belgium

Místo konání akce

Sanremo, Italy

Datum konání akce

1. 4. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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