Determination of self-incompatible genotypes in sweet cherry accessions of Czech genetic resources.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000096" target="_blank" >RIV/25271121:_____/19:N0000096 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/14864347:_____/19:N0000017

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.52" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1235.52</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1235.52" target="_blank" >10.17660/ActaHortic.2019.1235.52</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of self-incompatible genotypes in sweet cherry accessions of Czech genetic resources.

Popis výsledku v původním jazyce

Sweet cherries are self-incompatible determined by a gametophytic selfincompatibility system (GSI). The self-incompatibility is controlled by a multi-allelic Slocus and includes two genes coding for the synthesis of proteins responsible for the incompatibility response. The knowledge about the S-allele constitution of cultivars is very important for fruit growers and breeders. Recently, molecular methods have been developed to distinguish the S-alleles in sweet cherries and different molecular primers for the PCR-based identification of all S-alleles were designed during the last years. In our work, we analyzed S-locus genotypes by 10 universal and specific PCR primer combinations within 82 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. We confirmed previous S-genotyping for 46 accessions excepted Droganova, Hedelfingen, Erika Frühe Meckenheimer, Huldra, Rivan, Vanda and Winkler. It can be due to mislabelling or mistakes in previous analyses. Newly, S-genotyping was determined for 36 accessions when we found 4 new S-loci combinations. Totally, we detected S-locus combinations in 21 incompatibility group. The most frequent incompatibility groups were III (S3S4), IV (S2S3), VI (S3S6) and II (S1S3) with 13, 11, 9 and 8 genotypes, respectively.

Název v anglickém jazyce

Determination of self-incompatible genotypes in sweet cherry accessions of Czech genetic resources.

Popis výsledku anglicky

Sweet cherries are self-incompatible determined by a gametophytic selfincompatibility system (GSI). The self-incompatibility is controlled by a multi-allelic Slocus and includes two genes coding for the synthesis of proteins responsible for the incompatibility response. The knowledge about the S-allele constitution of cultivars is very important for fruit growers and breeders. Recently, molecular methods have been developed to distinguish the S-alleles in sweet cherries and different molecular primers for the PCR-based identification of all S-alleles were designed during the last years. In our work, we analyzed S-locus genotypes by 10 universal and specific PCR primer combinations within 82 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. We confirmed previous S-genotyping for 46 accessions excepted Droganova, Hedelfingen, Erika Frühe Meckenheimer, Huldra, Rivan, Vanda and Winkler. It can be due to mislabelling or mistakes in previous analyses. Newly, S-genotyping was determined for 36 accessions when we found 4 new S-loci combinations. Totally, we detected S-locus combinations in 21 incompatibility group. The most frequent incompatibility groups were III (S3S4), IV (S2S3), VI (S3S6) and II (S1S3) with 13, 11, 9 and 8 genotypes, respectively.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Projekt

<a href="/cs/project/QJ1510001" target="_blank" >QJ1510001: Výzkum genofondu třešně využitím molekulárně genetických metod</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

9789462612327

ISSN

—

e-ISSN

—

Počet stran výsledku

6

Strana od-do

379–385

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Yamagata, Japonsko

Datum konání akce

5. 6. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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