A rapid real-time PCR assay for detecting columnar growth in apples (Malus × domestica Borkh.)

Kód výsledku v IS VaVaI

RIV/25271121:_____/20:N0000127 - isvavai.cz

Výsledek na webu

https://onlinelibrary.wiley.com/doi/abs/10.1111/pbr.12861

DOI - Digital Object Identifier

10.1111/pbr.12861

Jazyk výsledku

angličtina

Název v původním jazyce

A rapid real-time PCR assay for detecting columnar growth in apples (Malus × domestica Borkh.)

Popis výsledku v původním jazyce

Columnar growth in apples can have considerable positive consequences for orchard maintenance, but the lack of columnar cultivars producing fruits with desired market quality prevents their broader utilization in orchards. New crossings focused on their breeding are therefore conducted throughout the world every year. Unfortunately, the reliable discrimination between columnar growth and normal phenotype is difficult until seedlings are 2-3 years of age. However, molecular marker assisted selection (MAS) can be used in seedlings several weeks old providing immense help in breeding programs focused on columnar apple production. Here, we describe a rapid and reliable method for the detection of both wild type (WT) and columnar (Co) alleles originating from ‘McIntosh Wijcik’ in a single reaction using a real-time PCR allelic discrimination approach. Our assay was tested in 130 genotypes (80 normal and 50 columnar habit) from various crossings and their parents, and in all cases, genotype corresponded to phenotype. The assay is thus suitable for laboratories interested in high-throughput MAS for columnar tree habit breeding.

Název v anglickém jazyce

A rapid real-time PCR assay for detecting columnar growth in apples (Malus × domestica Borkh.)

Popis výsledku anglicky

Columnar growth in apples can have considerable positive consequences for orchard maintenance, but the lack of columnar cultivars producing fruits with desired market quality prevents their broader utilization in orchards. New crossings focused on their breeding are therefore conducted throughout the world every year. Unfortunately, the reliable discrimination between columnar growth and normal phenotype is difficult until seedlings are 2-3 years of age. However, molecular marker assisted selection (MAS) can be used in seedlings several weeks old providing immense help in breeding programs focused on columnar apple production. Here, we describe a rapid and reliable method for the detection of both wild type (WT) and columnar (Co) alleles originating from ‘McIntosh Wijcik’ in a single reaction using a real-time PCR allelic discrimination approach. Our assay was tested in 130 genotypes (80 normal and 50 columnar habit) from various crossings and their parents, and in all cases, genotype corresponded to phenotype. The assay is thus suitable for laboratories interested in high-throughput MAS for columnar tree habit breeding.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

LO1608: Výzkumné ovocnářské centrum

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Breeding

ISSN

0179-9541

e-ISSN

—

Svazek periodika

139

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1244-1250

Kód UT WoS článku

000568901700001

EID výsledku v databázi Scopus

2-s2.0-85090942813