All S-alleles in one run: A new tool for the identification of S-alleles in sweet cherries

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F21%3AN0000042" target="_blank" >RIV/25271121:_____/21:N0000042 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

All S-alleles in one run: A new tool for the identification of S-alleles in sweet cherries

Popis výsledku v původním jazyce

This poster and short oral presentation introducing it were presented on the 1st International Symposium on Reproductive Biology of Fruit Tree Species, November 8 to November 10, 2021 -- entirely digital virtual format (https://symposium.inrae.fr/reproductive-biologyfruittree) . There was introduced a novel all-in-one kit enabling to identify all most frequent cherry S-alleles, including self-compatible ones in only one PCR and fragment analysis run. The kit covers the following alleles: S1, S2, S3, S4, S5, S6, S7, S9, S12, S13, S14, S22, S23, S24, S25, S28, S30, S34; and self-compatible alleles S3´, S4´, S5´. The kit also enables identification of MGST gene promoter insertion that is associated with cherry (partial) self-compatibility. This S-allele detection kit is highly specific and reliable, and saves labor, time and cost of the analysis. It can be used for routine S-allele identification, and it is especially suitable for analyses of cherries with unknown genotypes or free pollination selections.

Název v anglickém jazyce

All S-alleles in one run: A new tool for the identification of S-alleles in sweet cherries

Popis výsledku anglicky

This poster and short oral presentation introducing it were presented on the 1st International Symposium on Reproductive Biology of Fruit Tree Species, November 8 to November 10, 2021 -- entirely digital virtual format (https://symposium.inrae.fr/reproductive-biologyfruittree) . There was introduced a novel all-in-one kit enabling to identify all most frequent cherry S-alleles, including self-compatible ones in only one PCR and fragment analysis run. The kit covers the following alleles: S1, S2, S3, S4, S5, S6, S7, S9, S12, S13, S14, S22, S23, S24, S25, S28, S30, S34; and self-compatible alleles S3´, S4´, S5´. The kit also enables identification of MGST gene promoter insertion that is associated with cherry (partial) self-compatibility. This S-allele detection kit is highly specific and reliable, and saves labor, time and cost of the analysis. It can be used for routine S-allele identification, and it is especially suitable for analyses of cherries with unknown genotypes or free pollination selections.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/TN01000062" target="_blank" >TN01000062: Biotechnologické centrum pro genotypování rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů