Determination of sweet and sour cherry (in)compatibility in one PCR and fragment analysis reaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F23%3AN0000066" target="_blank" >RIV/25271121:_____/23:N0000066 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of sweet and sour cherry (in)compatibility in one PCR and fragment analysis reaction

Popis výsledku v původním jazyce

Presentation of a poster at the XVI EUCARPIA Symposium on Fruit Breeding and Genetics, Dresden-Pillnitz (Germany), 11.9.2023 - 16.9.2023. Both sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) possess so-called S-alleles determining their self-(in)compatibility. In case of self-incompatibility, suitably chosen pollinizers with distinct S-alleles have to be planted in orchards to ensure a high fruit set, however, harvest may be hampered by biotic and abiotic stresses that may influence successful pollination. Management of an orchard with two cultivars planted may be also complicated. Knowledge of S-alleles is therefore essential in all cultivated cherries, and self-compatible genotypes are obviously preferred. However, S-alleles determination is a challenging process run usually in several PCR and fragment analysis reactions as up-to-date 54 S-alleles have been identified in sweet cherries and 30 S-alleles in sour cherries. A novel kit has been developed to identify all known sweet cherry S-alleles including self-compatible ones in only one multiplex PCR and fragment analysis, and a similar kit for sour cherry is currently being optimized. Both assays are specific and reliable, enabling to distinguish also S-alleles differing by only one nucleotide mismatch (i.e. S5 vs S5´ in sweet cherry, S13 and S13´ in sour cherry). One-reaction analysis format is especially advantageous since it saves labor, time and cost of the analysis. The kits can be used for routine S-allele determination especially in unknown genotypes or free pollination selections or for marker-assisted selection of self-compatible genotypes.

Název v anglickém jazyce

Determination of sweet and sour cherry (in)compatibility in one PCR and fragment analysis reaction

Popis výsledku anglicky

Presentation of a poster at the XVI EUCARPIA Symposium on Fruit Breeding and Genetics, Dresden-Pillnitz (Germany), 11.9.2023 - 16.9.2023. Both sweet cherry (Prunus avium L.) and sour cherry (Prunus cerasus L.) possess so-called S-alleles determining their self-(in)compatibility. In case of self-incompatibility, suitably chosen pollinizers with distinct S-alleles have to be planted in orchards to ensure a high fruit set, however, harvest may be hampered by biotic and abiotic stresses that may influence successful pollination. Management of an orchard with two cultivars planted may be also complicated. Knowledge of S-alleles is therefore essential in all cultivated cherries, and self-compatible genotypes are obviously preferred. However, S-alleles determination is a challenging process run usually in several PCR and fragment analysis reactions as up-to-date 54 S-alleles have been identified in sweet cherries and 30 S-alleles in sour cherries. A novel kit has been developed to identify all known sweet cherry S-alleles including self-compatible ones in only one multiplex PCR and fragment analysis, and a similar kit for sour cherry is currently being optimized. Both assays are specific and reliable, enabling to distinguish also S-alleles differing by only one nucleotide mismatch (i.e. S5 vs S5´ in sweet cherry, S13 and S13´ in sour cherry). One-reaction analysis format is especially advantageous since it saves labor, time and cost of the analysis. The kits can be used for routine S-allele determination especially in unknown genotypes or free pollination selections or for marker-assisted selection of self-compatible genotypes.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40402 - GM technology (crops and livestock), livestock cloning, marker assisted selection, diagnostics (DNA chips and biosensing devices for the early/accurate detection of diseases) biomass feedstock production technologies, biopharming

Projekt

<a href="/cs/project/TN01000062" target="_blank" >TN01000062: Biotechnologické centrum pro genotypování rostlin</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů