Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F25%3AN0000009" target="_blank" >RIV/25328859:_____/25:N0000009 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41477-025-02110-w" target="_blank" >https://www.nature.com/articles/s41477-025-02110-w</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41477-025-02110-w" target="_blank" >10.1038/s41477-025-02110-w</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping

Popis výsledku v původním jazyce

Protecting crops from diseases is vital for the sustainable agricultural systems that are needed for food security. Introducing functional resistance genes to enhance the plant immune system is highly effective for disease resistance, but identifying new immune receptors is resource intensive. We observed that functional immune receptors of the nucleotide-binding domain leucine-rich repeat (NLR) class show a signature of high expression in uninfected plants across both monocot and dicot species. Here, by exploiting this signature combined with high-throughput transformation, we generated a wheat transgenic array of 995 NLRs from diverse grass species to identify new resistance genes for wheat. Confirming this proof of concept, we identified new resistance genes against the stem rust pathogen Puccinia graminis f. sp. tritici and the leaf rust pathogen Puccinia triticina, both major threats to wheat production. This pipeline facilitates the rapid identification of candidate NLRs and provides in planta gene validation of resistance. The accelerated discovery of new NLRs from a large gene pool of diverse and non-domesticated plant species will enhance the development of disease-resistant crops.

Název v anglickém jazyce

Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping

Popis výsledku anglicky

Protecting crops from diseases is vital for the sustainable agricultural systems that are needed for food security. Introducing functional resistance genes to enhance the plant immune system is highly effective for disease resistance, but identifying new immune receptors is resource intensive. We observed that functional immune receptors of the nucleotide-binding domain leucine-rich repeat (NLR) class show a signature of high expression in uninfected plants across both monocot and dicot species. Here, by exploiting this signature combined with high-throughput transformation, we generated a wheat transgenic array of 995 NLRs from diverse grass species to identify new resistance genes for wheat. Confirming this proof of concept, we identified new resistance genes against the stem rust pathogen Puccinia graminis f. sp. tritici and the leaf rust pathogen Puccinia triticina, both major threats to wheat production. This pipeline facilitates the rapid identification of candidate NLRs and provides in planta gene validation of resistance. The accelerated discovery of new NLRs from a large gene pool of diverse and non-domesticated plant species will enhance the development of disease-resistant crops.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Plants

ISSN

2055-026X

e-ISSN

2055-0278

Svazek periodika

11

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

15

Strana od-do

2100-2114

Kód UT WoS článku

001576816900001

EID výsledku v databázi Scopus

2-s2.0-105016887175