NLRSEEK: High-Throughput Discovery Pipeline for Functional Resistance Genes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25328859%3A_____%2F23%3AN0000022" target="_blank" >RIV/25328859:_____/23:N0000022 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

NLRSEEK: High-Throughput Discovery Pipeline for Functional Resistance Genes

Popis výsledku v původním jazyce

Breeding crop species that are safe from pests and diseases is vital to build sustainable food systems crucial for food security. An effective and environmentally friendly method of disease control is to enhance the plant immune system by introducing functional resistance genes. A major class of plant immune receptors are nucleotide-binding, leucine-rich repeat receptors (NLRs), however identifying NLRs for use in elite crop varieties is time-consuming and resource-intensive. Through analyses of copy number variation and expression data, we identify high expression as an overlooked molecular signature of functional NLRs. Combining this signature with high-throughput crop transformation, we developed an approach that enables rapid identification and in planta validation of NLRs from non-domesticated germplasm. Screening 995 NLRs from 18 grass species identified 19 new resistance genes against wheat stem rust, a critical threat to wheat production. This pipeline facilitates rapid resistance gene discovery from diverse plant species to generate disease-resistant crops. Poster at: 12th International Congress of Plant Pathology (ICPP) 2023. One Health for all plants, crops and trees. 20-25 August, France. Book of Abstracts, 12, 2023, K6-3, 11.

Název v anglickém jazyce

NLRSEEK: High-Throughput Discovery Pipeline for Functional Resistance Genes

Popis výsledku anglicky

Breeding crop species that are safe from pests and diseases is vital to build sustainable food systems crucial for food security. An effective and environmentally friendly method of disease control is to enhance the plant immune system by introducing functional resistance genes. A major class of plant immune receptors are nucleotide-binding, leucine-rich repeat receptors (NLRs), however identifying NLRs for use in elite crop varieties is time-consuming and resource-intensive. Through analyses of copy number variation and expression data, we identify high expression as an overlooked molecular signature of functional NLRs. Combining this signature with high-throughput crop transformation, we developed an approach that enables rapid identification and in planta validation of NLRs from non-domesticated germplasm. Screening 995 NLRs from 18 grass species identified 19 new resistance genes against wheat stem rust, a critical threat to wheat production. This pipeline facilitates rapid resistance gene discovery from diverse plant species to generate disease-resistant crops. Poster at: 12th International Congress of Plant Pathology (ICPP) 2023. One Health for all plants, crops and trees. 20-25 August, France. Book of Abstracts, 12, 2023, K6-3, 11.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů