Pm6 from Triticum timopheevii encodes an NLR receptor that directly recognizes AvrPm6 to confer powdery mildew resistance in wheat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00642573" target="_blank" >RIV/61389030:_____/25:00642573 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.molp.2025.10.012" target="_blank" >https://doi.org/10.1016/j.molp.2025.10.012</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.molp.2025.10.012" target="_blank" >10.1016/j.molp.2025.10.012</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Pm6 from Triticum timopheevii encodes an NLR receptor that directly recognizes AvrPm6 to confer powdery mildew resistance in wheat

Popis výsledku v původním jazyce

The study reports the successful cloning and functional characterization of the wheat powdery mildew resistance gene Pm6, originating from Triticum timopheevii. Using map-based cloning, chromosome sorting, EMS mutagenesis, and transcriptomic analysis, the authors identify TraesLAC2B03G00988960 as the gene underlying Pm6, encoding an NLR-type immune receptor with a C-terminal PRK domain. The research further reveals the corresponding fungal effector AvrPm6, identified through genome-wide association analysis of Blumeria graminis f. sp. tritici isolates. Biochemical assays demonstrate a direct physical interaction between Pm6 and AvrPm6, which triggers immune activation, reactive oxygen species accumulation, and cell death. Functional validation through gene silencing, overexpression, and gene-editing confirms that Pm6-mediated resistance is strictly dependent on this AvrPm6 recognition. The findings elucidate a previously unknown effector–receptor pair in wheat immunity and provide valuable resources for resistance breeding and engineering of durable disease resistance.

Název v anglickém jazyce

Pm6 from Triticum timopheevii encodes an NLR receptor that directly recognizes AvrPm6 to confer powdery mildew resistance in wheat

Popis výsledku anglicky

The study reports the successful cloning and functional characterization of the wheat powdery mildew resistance gene Pm6, originating from Triticum timopheevii. Using map-based cloning, chromosome sorting, EMS mutagenesis, and transcriptomic analysis, the authors identify TraesLAC2B03G00988960 as the gene underlying Pm6, encoding an NLR-type immune receptor with a C-terminal PRK domain. The research further reveals the corresponding fungal effector AvrPm6, identified through genome-wide association analysis of Blumeria graminis f. sp. tritici isolates. Biochemical assays demonstrate a direct physical interaction between Pm6 and AvrPm6, which triggers immune activation, reactive oxygen species accumulation, and cell death. Functional validation through gene silencing, overexpression, and gene-editing confirms that Pm6-mediated resistance is strictly dependent on this AvrPm6 recognition. The findings elucidate a previously unknown effector–receptor pair in wheat immunity and provide valuable resources for resistance breeding and engineering of durable disease resistance.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Plant

ISSN

1674-2052

e-ISSN

1752-9867

Svazek periodika

18

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

2040-2044

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-105022901666