Multiple Selection of Red Clover Based on Symbiotic Nitrogen Fixation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26296080%3A_____%2F25%3AN0000049" target="_blank" >RIV/26296080:_____/25:N0000049 - isvavai.cz</a>

Výsledek na webu

<a href="https://39533ef9f9.clvaw-cdnwnd.com/a3143b19de5ac9ac6a632f80232e9418/200000356-b9825b9827/Eucarpia_FCAG_2025_Abstracts.pdf?ph=39533ef9f9" target="_blank" >https://39533ef9f9.clvaw-cdnwnd.com/a3143b19de5ac9ac6a632f80232e9418/200000356-b9825b9827/Eucarpia_FCAG_2025_Abstracts.pdf?ph=39533ef9f9</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Multiple Selection of Red Clover Based on Symbiotic Nitrogen Fixation

Popis výsledku v původním jazyce

Current trends in agriculture are shifting towards sustainable and ecological farming systems that aim to reduce dependence on synthetic inputs, particularly industrial nitrogen fertilizers. One of the key strategies to achieve this goal is the utilization of natural biological nitrogen fixation (BNF), a process carried out by red clover (Trifolium pratense L.) and other leguminous plants in symbiosis with root-nodulating bacteria of the order Rhizobiales. Red clover is a cross-pollinated species characterized by considerable intraspecific variability, which includes variation in the ability of individual genotypes to fix atmospheric nitrogen. Our study focuses on the recurrent selection of red clover genotypes with the aim of enhancing their BNF capacity. The selection of beneficial plants was based on measurements of nitrogenase enzyme activity using the acetylene reduction assay (ARA). Four selection cycles were conducted. In each cycle, plants with the highest nitrogenase activity were selected, intercrossed, and their progeny were subsequently re-evaluated using the ARA method. The final evaluation of progeny, along with comparison to parental population and the initial population, was conducted in 2025. The results indicate that during each selection year there was a noticeable increasing trend of nitrogen fixation and the trait variability was lower compared to parental populations. These findings are consistent with the assumption that nitrogen fixation is a quantitative trait governed by polygenic inheritance. The resulting plant material represents a valuable resource for the development of new cultivars with improved and more stable nitrogen fixation capacity.

Název v anglickém jazyce

Multiple Selection of Red Clover Based on Symbiotic Nitrogen Fixation

Popis výsledku anglicky

Current trends in agriculture are shifting towards sustainable and ecological farming systems that aim to reduce dependence on synthetic inputs, particularly industrial nitrogen fertilizers. One of the key strategies to achieve this goal is the utilization of natural biological nitrogen fixation (BNF), a process carried out by red clover (Trifolium pratense L.) and other leguminous plants in symbiosis with root-nodulating bacteria of the order Rhizobiales. Red clover is a cross-pollinated species characterized by considerable intraspecific variability, which includes variation in the ability of individual genotypes to fix atmospheric nitrogen. Our study focuses on the recurrent selection of red clover genotypes with the aim of enhancing their BNF capacity. The selection of beneficial plants was based on measurements of nitrogenase enzyme activity using the acetylene reduction assay (ARA). Four selection cycles were conducted. In each cycle, plants with the highest nitrogenase activity were selected, intercrossed, and their progeny were subsequently re-evaluated using the ARA method. The final evaluation of progeny, along with comparison to parental population and the initial population, was conducted in 2025. The results indicate that during each selection year there was a noticeable increasing trend of nitrogen fixation and the trait variability was lower compared to parental populations. These findings are consistent with the assumption that nitrogen fixation is a quantitative trait governed by polygenic inheritance. The resulting plant material represents a valuable resource for the development of new cultivars with improved and more stable nitrogen fixation capacity.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů