Selected factors influencing the ability of Bifidobacterium to form biogenic amines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F14%3A%23TA82" target="_blank" >RIV/26722861:_____/14:#TA82 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/ijfs.12427/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/ijfs.12427/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ijfs.12427" target="_blank" >10.1111/ijfs.12427</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Selected factors influencing the ability of Bifidobacterium to form biogenic amines

Popis výsledku v původním jazyce

The ability of typical probiotic culture of Bifidobacterium to produce biogenic amines could be considered a contrastive feature to the beneficial dietary effect on human health. The aim of this pilot study was to evaluate the decarboxylase activity of Bifidobacterium animalis subsp. lactis CCDM 239 influenced by selected factors (pH 4.5 and 5.0; the contents of NaCl 0–20.0 g L 1, glucose and lactose 0–10.0 g L 1) at in vitro conditions. The kinetics of the biogenic amine production under the above-mentioned conditions was also monitored. The biogenic amine content detection was carried out in the supernatants of inoculated broth [MRS enriched with amino acids: arginine, ornithine, lysine, tyrosine; 3 g L 1 after the cultivation (48 h, 37 1 °C)]. RP-HPLC after the precolumn derivatisation with dansyl chloride was used. In most cases, the low concentrations of tyramine were monitored (<15 mg L 1). Simultaneously, it was found out that the addition of certain fermentable saccharide concentrations and NaCl in their mutual combination seemed to have supporting effect on the decarboxylase activity of the tested Bifidobacterium.

Název v anglickém jazyce

Selected factors influencing the ability of Bifidobacterium to form biogenic amines

Popis výsledku anglicky

The ability of typical probiotic culture of Bifidobacterium to produce biogenic amines could be considered a contrastive feature to the beneficial dietary effect on human health. The aim of this pilot study was to evaluate the decarboxylase activity of Bifidobacterium animalis subsp. lactis CCDM 239 influenced by selected factors (pH 4.5 and 5.0; the contents of NaCl 0–20.0 g L 1, glucose and lactose 0–10.0 g L 1) at in vitro conditions. The kinetics of the biogenic amine production under the above-mentioned conditions was also monitored. The biogenic amine content detection was carried out in the supernatants of inoculated broth [MRS enriched with amino acids: arginine, ornithine, lysine, tyrosine; 3 g L 1 after the cultivation (48 h, 37 1 °C)]. RP-HPLC after the precolumn derivatisation with dansyl chloride was used. In most cases, the low concentrations of tyramine were monitored (<15 mg L 1). Simultaneously, it was found out that the addition of certain fermentable saccharide concentrations and NaCl in their mutual combination seemed to have supporting effect on the decarboxylase activity of the tested Bifidobacterium.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Food Science and Technology

ISSN

1365-2621

e-ISSN

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Svazek periodika

49

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

1302-1307

Kód UT WoS článku

000334079700009

EID výsledku v databázi Scopus

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