Effects of temperature, pH and NaCl content on in vitro putrescine and cadaverine production through the growth of Serratia marcescens CCM 303

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28110%2F15%3A43873250" target="_blank" >RIV/70883521:28110/15:43873250 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/03601234.2015.1058097" target="_blank" >http://dx.doi.org/10.1080/03601234.2015.1058097</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/03601234.2015.1058097" target="_blank" >10.1080/03601234.2015.1058097</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of temperature, pH and NaCl content on in vitro putrescine and cadaverine production through the growth of Serratia marcescens CCM 303

Popis výsledku v původním jazyce

The aim of this study was to evaluate the combined effect of temperature (10, 20 and 37 degrees C), pH (4, 5, 6, 7 and 8), and NaCl content (0, 1, 3, 4, 5 and 6% w/v) on the growth and putrescine and cadaverine production of Serratia marcescens CCM 303 under model conditions. The decarboxylase activity of S. marcescens was monitored in broth after cultivation. The cultivation medium was enriched with selected amino acids (ornithine, arginine and lysine; 0.2% w/v each) serving as precursors of biogenic amines. Levels of putrescine and cadaverine in broth were analysed by high-performance liquid chromatography after pre-column derivatisation with o-phthalaldehyde reagent. S. marcescens produced higher amounts of putrescine (up to 2096.8mg L-1) compared to cadaverine content (up to 343.3mg L-1) in all cultivation media. The highest putrescine and cadaverine concentrations were reached during cultivation at 10-20 degrees C, pH 5-7 and NaCl content 1-3% w/v. On the other hand, the highest B

Název v anglickém jazyce

Effects of temperature, pH and NaCl content on in vitro putrescine and cadaverine production through the growth of Serratia marcescens CCM 303

Popis výsledku anglicky

The aim of this study was to evaluate the combined effect of temperature (10, 20 and 37 degrees C), pH (4, 5, 6, 7 and 8), and NaCl content (0, 1, 3, 4, 5 and 6% w/v) on the growth and putrescine and cadaverine production of Serratia marcescens CCM 303 under model conditions. The decarboxylase activity of S. marcescens was monitored in broth after cultivation. The cultivation medium was enriched with selected amino acids (ornithine, arginine and lysine; 0.2% w/v each) serving as precursors of biogenic amines. Levels of putrescine and cadaverine in broth were analysed by high-performance liquid chromatography after pre-column derivatisation with o-phthalaldehyde reagent. S. marcescens produced higher amounts of putrescine (up to 2096.8mg L-1) compared to cadaverine content (up to 343.3mg L-1) in all cultivation media. The highest putrescine and cadaverine concentrations were reached during cultivation at 10-20 degrees C, pH 5-7 and NaCl content 1-3% w/v. On the other hand, the highest B

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/GAP503%2F11%2F1417" target="_blank" >GAP503/11/1417: Produkce biogenních aminů u vybraných kmenů bakterií mléčného kvašení</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes

ISSN

0360-1234

e-ISSN

—

Svazek periodika

50

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

797-808

Kód UT WoS článku

000361121600005

EID výsledku v databázi Scopus

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