Polymorphism and association of the FASN gene with milk production traits in czech sheep population

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F18%3AN0000081" target="_blank" >RIV/26722861:_____/18:N0000081 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027014:_____/18:N0000289

Výsledek na webu

<a href="http://www.jhed.mk/filemanager/JHED%20Vol.%2024/03.%20FPP/09.%20Full%20paper%20-%20Zuzana%20Sztankoova.pdf" target="_blank" >http://www.jhed.mk/filemanager/JHED%20Vol.%2024/03.%20FPP/09.%20Full%20paper%20-%20Zuzana%20Sztankoova.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polymorphism and association of the FASN gene with milk production traits in czech sheep population

Popis výsledku v původním jazyce

The objective of this study was to identify single nucleotide polymorphism (SNP) of the ovine fatty acid synthase (FASN) gene and to evaluate its effect on milk parameters and fatty acids (FAs) composition in sheep milk. The FASN gene encodes an enzyme cata-lysing the de novo synthesis of FAs in cells and is mainly expressed in adipose tissue. Milk FA profiles play an important role in dairy sheep farming because they have a large effect on cheese characteristics and also because sheep milk may be designated as a source of nutraceuticals inasmuch as it contains higher levels of conjugated linoleic acid than does milk from other ruminants. Typing SNP within exon 32 of FASN at positon g.257C>T was performed using the rapid and efficient TaqMan assay method. The study was performed for 772 sheep kept in the Czech Republic and belonging to the following breeds: East Friesian (529 animals), Lacaune (152 animals), Romanov (51 animals), and Valachian (40 animals). We used 507 animals with 8593 dairy production records to evaluate the effect of genetic polymorphism at the FASN locus on milk parameters. To determine allele frequency, genotype distribution, observed (Ho) and expected (He) heterozygosity as well as to verify Hardy–Weinberg equilibrium was used GenAlEx 6.502 analysis. Analysis of variance was carrying out using the MIXED model procedure in SAS software v. 9.4. A two different model equation was chosen for testing performance data depending upon which trait was being evaluated. To determine the effect of genetic polymorphism on milk parameters was used followed model: the fixed effects were genotype, herd-year of performance, days in milk (DIM), DIM*DIM, month of performance, and parity number. Molecular analysis showed prevalence of the C allele (81.67%) compared to the T allele (18.33%). The dominant genotype was CC (43%-85%), followed CT (15%-47%) and TT (0%-16%) depending on breed and number of animals observed. All four breeds were in Har-dy-Weinberg equilibrium (P < 0.05) and showed weak heterozygosity at the FASN locus. The SNP g.257C>T within exon 32 of FASN was significantly associated with protein, fat, and lactose percentage. For FA analysis, we used 94 individuals with 112 dairy records. To determine the effect of genetic polymorphism on fatty acids was used followed model: a fixed effect was number of lactation, genotype and year of sample collection. The animal genetic effect was set as the random effect (repeated measurements per sheep). The animal genetic effect was set as the random effect (repeated measurements per sheep). Statistical analysis confirmed association with the minority FA components: valeric acid (C5:0) and pentadecylic acid (C15:0), and a tendency was observed for association with oleic acid (C18:1n9c) and gamma-linoleic acid (C18:3n6) (P < 0.05). In conclusion, the SNP g.257C>T contributes to influence on the medium-and long-chain FAs. FASN is a candidate gene for milk parameters and may be used as a genetic marker for improving milk quality and milk fat content in sheep.

Název v anglickém jazyce

Polymorphism and association of the FASN gene with milk production traits in czech sheep population

Popis výsledku anglicky

The objective of this study was to identify single nucleotide polymorphism (SNP) of the ovine fatty acid synthase (FASN) gene and to evaluate its effect on milk parameters and fatty acids (FAs) composition in sheep milk. The FASN gene encodes an enzyme cata-lysing the de novo synthesis of FAs in cells and is mainly expressed in adipose tissue. Milk FA profiles play an important role in dairy sheep farming because they have a large effect on cheese characteristics and also because sheep milk may be designated as a source of nutraceuticals inasmuch as it contains higher levels of conjugated linoleic acid than does milk from other ruminants. Typing SNP within exon 32 of FASN at positon g.257C>T was performed using the rapid and efficient TaqMan assay method. The study was performed for 772 sheep kept in the Czech Republic and belonging to the following breeds: East Friesian (529 animals), Lacaune (152 animals), Romanov (51 animals), and Valachian (40 animals). We used 507 animals with 8593 dairy production records to evaluate the effect of genetic polymorphism at the FASN locus on milk parameters. To determine allele frequency, genotype distribution, observed (Ho) and expected (He) heterozygosity as well as to verify Hardy–Weinberg equilibrium was used GenAlEx 6.502 analysis. Analysis of variance was carrying out using the MIXED model procedure in SAS software v. 9.4. A two different model equation was chosen for testing performance data depending upon which trait was being evaluated. To determine the effect of genetic polymorphism on milk parameters was used followed model: the fixed effects were genotype, herd-year of performance, days in milk (DIM), DIM*DIM, month of performance, and parity number. Molecular analysis showed prevalence of the C allele (81.67%) compared to the T allele (18.33%). The dominant genotype was CC (43%-85%), followed CT (15%-47%) and TT (0%-16%) depending on breed and number of animals observed. All four breeds were in Har-dy-Weinberg equilibrium (P < 0.05) and showed weak heterozygosity at the FASN locus. The SNP g.257C>T within exon 32 of FASN was significantly associated with protein, fat, and lactose percentage. For FA analysis, we used 94 individuals with 112 dairy records. To determine the effect of genetic polymorphism on fatty acids was used followed model: a fixed effect was number of lactation, genotype and year of sample collection. The animal genetic effect was set as the random effect (repeated measurements per sheep). The animal genetic effect was set as the random effect (repeated measurements per sheep). Statistical analysis confirmed association with the minority FA components: valeric acid (C5:0) and pentadecylic acid (C15:0), and a tendency was observed for association with oleic acid (C18:1n9c) and gamma-linoleic acid (C18:3n6) (P < 0.05). In conclusion, the SNP g.257C>T contributes to influence on the medium-and long-chain FAs. FASN is a candidate gene for milk parameters and may be used as a genetic marker for improving milk quality and milk fat content in sheep.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

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OECD FORD obor

40203 - Husbandry

Projekt

<a href="/cs/project/QJ1310107" target="_blank" >QJ1310107: Vliv genetického polymorfizmu lipogenických enzymů na složení mléčného tuku a obsah mastných kyselin v mléce malých přežvýkavců (kozy a ovce).</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Hygienic Engineering and Design

ISSN

1857-8489

e-ISSN

1857-8489

Svazek periodika

24

Číslo periodika v rámci svazku

18

Stát vydavatele periodika

MK - Republika Severní Makedonie

Počet stran výsledku

6

Strana od-do

84 - 89

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-85059409332