The occurrence of common species of the opportunistic fungal pathogens in dairies and bioptic samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F19%3AN0000062" target="_blank" >RIV/26722861:_____/19:N0000062 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The occurrence of common species of the opportunistic fungal pathogens in dairies and bioptic samples

Popis výsledku v původním jazyce

PP prezentace and Abstract The 2019 ICFM Conference on “Food- and Airborne Fungi – Challenges for Food Safety and Supply” Freising, Germany on June 03 - 05, 2019, p 5 – 6. The opportunistic fungal pathogens (OFP) are represented by fungal species that follow a wide spectrum of life strategies and inhabit various ecological niches. The OFP in dairy operations causes severe problems in spite of massive preventive measures. Contamination of products results in economic loss but also pose a health risk for workers and consumers. The objectives of this study were 1. To survey the occurrence of OFP in the environment and in products in HCCP dairies and family farm dairies and 2. To evaluate the OFP as causal agents of mycoses. The fungal contaminants were isolated and identified from the environment concerning their adaptability to the extreme conditions in dairy operations such as salt bath, pasteurization, temperature, sanitation. The microbial and fungal diversity of milk products from pasteurised and non-pasteurised milk were compared. The fungal isolates were analysed by using molecular methods and sequencing combined with classical microbiological methods. The anonymous bioptic samples were analysed based on the type of immunodeficiency and disease etiology to obtain the current status about the pathogenicity of OFP for immunocompromised individuals. NGS methods and DNA barcoding were further analyzed these samples to assign individual OFPs to the relevant pathological findings. The results showed that many OFP identified in pulmonary, dermatological and colon bioptic samples such as Aspergillus nigricans, Cladosporium spp., Alternaria spp., Fusarium spp. are also representatives of dairy contaminants. These species have a high adaptability and resistance to the extreme environment, antibiotics, and sanitation. The fungal diversity between HACCP dairies and farm dairies was found to be significantly different. The prevalence of Penicillium-species and yeast contaminants was found in farm samples whereas the occurrence of OFP contaminants resistant to sanitary chemicals was noted in HACCP dairies first at all.

Název v anglickém jazyce

The occurrence of common species of the opportunistic fungal pathogens in dairies and bioptic samples

Popis výsledku anglicky

PP prezentace and Abstract The 2019 ICFM Conference on “Food- and Airborne Fungi – Challenges for Food Safety and Supply” Freising, Germany on June 03 - 05, 2019, p 5 – 6. The opportunistic fungal pathogens (OFP) are represented by fungal species that follow a wide spectrum of life strategies and inhabit various ecological niches. The OFP in dairy operations causes severe problems in spite of massive preventive measures. Contamination of products results in economic loss but also pose a health risk for workers and consumers. The objectives of this study were 1. To survey the occurrence of OFP in the environment and in products in HCCP dairies and family farm dairies and 2. To evaluate the OFP as causal agents of mycoses. The fungal contaminants were isolated and identified from the environment concerning their adaptability to the extreme conditions in dairy operations such as salt bath, pasteurization, temperature, sanitation. The microbial and fungal diversity of milk products from pasteurised and non-pasteurised milk were compared. The fungal isolates were analysed by using molecular methods and sequencing combined with classical microbiological methods. The anonymous bioptic samples were analysed based on the type of immunodeficiency and disease etiology to obtain the current status about the pathogenicity of OFP for immunocompromised individuals. NGS methods and DNA barcoding were further analyzed these samples to assign individual OFPs to the relevant pathological findings. The results showed that many OFP identified in pulmonary, dermatological and colon bioptic samples such as Aspergillus nigricans, Cladosporium spp., Alternaria spp., Fusarium spp. are also representatives of dairy contaminants. These species have a high adaptability and resistance to the extreme environment, antibiotics, and sanitation. The fungal diversity between HACCP dairies and farm dairies was found to be significantly different. The prevalence of Penicillium-species and yeast contaminants was found in farm samples whereas the occurrence of OFP contaminants resistant to sanitary chemicals was noted in HACCP dairies first at all.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QK1910036" target="_blank" >QK1910036: Využití potenciálu různých metabolitů divokých kmenů bakterií mléčného kvašení a kvasinek k potlačení výskytu nežádoucích fungálních kontaminantů a prodloužení trvanlivosti mléčných a pekařských výrobků.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů