The identification of rumen ciliates using optimized Cryo-SEM approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26788462%3A_____%2F24%3AN0000042" target="_blank" >RIV/26788462:_____/24:N0000042 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/26788462:_____/24:N0000041

Výsledek na webu

<a href="https://www.agrovyzkum.cz/soubory/bulletin/2024/1/bull_1_2024_25-31_malyugina.pdf" target="_blank" >https://www.agrovyzkum.cz/soubory/bulletin/2024/1/bull_1_2024_25-31_malyugina.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The identification of rumen ciliates using optimized Cryo-SEM approach

Popis výsledku v původním jazyce

The rumen is inhabited by a dense and highly diverse microbial community, consisting of bacteria, archaea, protozoa and fungi. Feeds consumed by animals are digested with the help of rumen microorganisms, which play an important role in contributing nutrients to the host animal. The present study aims to determine and investigate protozoal ciliate species in the rumen using modern microscopy and propose a new method of rumen microorganism observation and compare it with the classical microscopic method. For these purposes, fresh ruminal liquor was collected from four cannulated cows housed in individual pens. The animals were fed the high-concentrated basal diet consisting of 65.82 % hay, 48.06 % grass silage, and 16.82 % cereal concentrate enriched with minerals and vitamins. The experimental group consisted of three cows whose diet was enriched with 30 g (3.14 g/kg of DM) of lyophilized microalgae Chlorella Vulgaris. Total daily dry matter intake per feeding was 11.9 kg. Rumen liquor samples were collected three hours after the morning feeding twice a week via a rumen cannula with a probe connected to a vacuum pump. After the collection, aliquot samples were immediately transported to the laboratory for further analysis. In this article, we included results of observing protozoal ciliates and their ultrastructures obtained with the optimized cryo-scanning electron microscopy method.

Název v anglickém jazyce

The identification of rumen ciliates using optimized Cryo-SEM approach

Popis výsledku anglicky

The rumen is inhabited by a dense and highly diverse microbial community, consisting of bacteria, archaea, protozoa and fungi. Feeds consumed by animals are digested with the help of rumen microorganisms, which play an important role in contributing nutrients to the host animal. The present study aims to determine and investigate protozoal ciliate species in the rumen using modern microscopy and propose a new method of rumen microorganism observation and compare it with the classical microscopic method. For these purposes, fresh ruminal liquor was collected from four cannulated cows housed in individual pens. The animals were fed the high-concentrated basal diet consisting of 65.82 % hay, 48.06 % grass silage, and 16.82 % cereal concentrate enriched with minerals and vitamins. The experimental group consisted of three cows whose diet was enriched with 30 g (3.14 g/kg of DM) of lyophilized microalgae Chlorella Vulgaris. Total daily dry matter intake per feeding was 11.9 kg. Rumen liquor samples were collected three hours after the morning feeding twice a week via a rumen cannula with a probe connected to a vacuum pump. After the collection, aliquot samples were immediately transported to the laboratory for further analysis. In this article, we included results of observing protozoal ciliates and their ultrastructures obtained with the optimized cryo-scanning electron microscopy method.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/SS06020190" target="_blank" >SS06020190: Vývoj antimetanogenního krmného suplementu pro zmírnění environmentálního dopadu z chovu hospodářských zvířat</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Výzkum v chovu skotu

ISSN

0139-7265

e-ISSN

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Svazek periodika

244

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

25-31

Kód UT WoS článku

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EID výsledku v databázi Scopus

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