Distinguishing of Francisella tularensis subsp. tularensis and Francisella tularensis subsp. holarctica based on SILAC relative quantification of differentially expressed gene products
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27093123%3A_____%2F14%3A%230000002" target="_blank" >RIV/27093123:_____/14:#0000002 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Distinguishing of Francisella tularensis subsp. tularensis and Francisella tularensis subsp. holarctica based on SILAC relative quantification of differentially expressed gene products
Popis výsledku v původním jazyce
Utilizing SILAC quantification approach and high resolution mass spectrometry we monitor the differences in membrane proteome of Francisella tularensis subsp. tularensis and Francisella tularensis subsp. holarctica. Even these subspecies differ by 85% ongenome level we were able successfully quantify over 500 hundreds proteins. Based on quantification study we selected: eleven proteins that are uniquely expressed in Francisella tularensis subsp. tularensis or Francisella tularensis subsp. holarctica and 32 proteins differing in expression level. Subsequently we expressed these proteins in E. coli and used them to produce rabbit polyclonal antibodies. We confirmed our SILAC results and antibody specificity using combination of peptide mass fingerprinting and western blots.
Název v anglickém jazyce
Distinguishing of Francisella tularensis subsp. tularensis and Francisella tularensis subsp. holarctica based on SILAC relative quantification of differentially expressed gene products
Popis výsledku anglicky
Utilizing SILAC quantification approach and high resolution mass spectrometry we monitor the differences in membrane proteome of Francisella tularensis subsp. tularensis and Francisella tularensis subsp. holarctica. Even these subspecies differ by 85% ongenome level we were able successfully quantify over 500 hundreds proteins. Based on quantification study we selected: eleven proteins that are uniquely expressed in Francisella tularensis subsp. tularensis or Francisella tularensis subsp. holarctica and 32 proteins differing in expression level. Subsequently we expressed these proteins in E. coli and used them to produce rabbit polyclonal antibodies. We confirmed our SILAC results and antibody specificity using combination of peptide mass fingerprinting and western blots.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/FR-TI1%2F292" target="_blank" >FR-TI1/292: Molekulární diagnostika bakteriálních patogenů</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů