Preservation of urine specimens for metabolic evaluation of recurrent urinary stone formers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F27661989%3A_____%2F25%3A10002847" target="_blank" >RIV/27661989:_____/25:10002847 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/25:10488697

Výsledek na webu

<a href="https://www.degruyterbrill.com/document/doi/10.1515/cclm-2024-0773/html?srsltid=AfmBOoodGbOqkCpJ0bowC-gZ6gBF_hx5e36rFC6fTQuSbL0F0NCleD6M" target="_blank" >https://www.degruyterbrill.com/document/doi/10.1515/cclm-2024-0773/html?srsltid=AfmBOoodGbOqkCpJ0bowC-gZ6gBF_hx5e36rFC6fTQuSbL0F0NCleD6M</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/cclm-2024-0773" target="_blank" >10.1515/cclm-2024-0773</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preservation of urine specimens for metabolic evaluation of recurrent urinary stone formers

Popis výsledku v původním jazyce

Objectives Stability of concentrations of urinary stone-related metabolites was analyzed from samples of recurrent urinary stone formers to assess necessity and effectiveness of urine acidification during collection and storage.Methods First-morning urine was collected from 20 adult calcium-stone forming patients at Tomas Bata Hospital in the Czech Republic. Urine samples were analyzed for calcium, magnesium, inorganic phosphate, uric acid, sodium, potassium, chloride, citrate, oxalate, and urine particles. The single-voided specimens were collected without acidification, after which they were divided into three groups for storage: samples without acidification (&quot;NON&quot;), acidification before storage (&quot;PRE&quot;), or acidification after storage (&quot;POST&quot;). The analyses were conducted on the day of arrival (day 0, &quot;baseline&quot;), or after storage for 2 or 7 days at room temperature. The maximum permissible difference (MPD) was defined as +/- 20 % from the baseline.Results The urine concentrations of all stone-related metabolites remained within the 20 % MPD limits in NON and POST samples after 2 days, except for calcium in NON sample of one patient, and oxalate of three patients and citrate of one patient in POST samples. In PRE samples, stability failed in urine samples for oxalate of three patients, and for uric acid of four patients after 2 days. Failures in stability often correlated with high baseline concentrations of those metabolites in urine.Conclusions Detailed procedures are needed to collect urine specimens for analysis of urinary stone-related metabolites, considering both patient safety and stability of those metabolites. We recommend specific preservation steps.

Název v anglickém jazyce

Preservation of urine specimens for metabolic evaluation of recurrent urinary stone formers

Popis výsledku anglicky

Objectives Stability of concentrations of urinary stone-related metabolites was analyzed from samples of recurrent urinary stone formers to assess necessity and effectiveness of urine acidification during collection and storage.Methods First-morning urine was collected from 20 adult calcium-stone forming patients at Tomas Bata Hospital in the Czech Republic. Urine samples were analyzed for calcium, magnesium, inorganic phosphate, uric acid, sodium, potassium, chloride, citrate, oxalate, and urine particles. The single-voided specimens were collected without acidification, after which they were divided into three groups for storage: samples without acidification (&quot;NON&quot;), acidification before storage (&quot;PRE&quot;), or acidification after storage (&quot;POST&quot;). The analyses were conducted on the day of arrival (day 0, &quot;baseline&quot;), or after storage for 2 or 7 days at room temperature. The maximum permissible difference (MPD) was defined as +/- 20 % from the baseline.Results The urine concentrations of all stone-related metabolites remained within the 20 % MPD limits in NON and POST samples after 2 days, except for calcium in NON sample of one patient, and oxalate of three patients and citrate of one patient in POST samples. In PRE samples, stability failed in urine samples for oxalate of three patients, and for uric acid of four patients after 2 days. Failures in stability often correlated with high baseline concentrations of those metabolites in urine.Conclusions Detailed procedures are needed to collect urine specimens for analysis of urinary stone-related metabolites, considering both patient safety and stability of those metabolites. We recommend specific preservation steps.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Clinical Chemistry and Laboratory Medicine

ISSN

1434-6621

e-ISSN

1437-4331

Svazek periodika

63

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

129-138

Kód UT WoS článku

001278957200001

EID výsledku v databázi Scopus

2-s2.0-85200157841