Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F18%3A43893676" target="_blank" >RIV/44555601:13440/18:43893676 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0927776517308494?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0927776517308494?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.12.009" target="_blank" >10.1016/j.colsurfb.2017.12.009</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions

Popis výsledku v původním jazyce

tThe interaction of a double stranded small interference RNA (siRNA Nef) with cationic carbosilane den-drimers of generations 1-3 with two different ammonium functions at the periphery ([MINUS SIGN NMe2R]+, R = Me,(CH2)2OH) has been studied by experimental techniques (zeta potential, electrophoresis, single moleculepulling experiments) and molecular dynamic calculations. These studies state the presence of differentforces on dendriplex formation, depending on generation and type of ammonium group. Whilst for higherdendrimers electrostatic forces mainly drive the stability of dendriplexes, first generation compoundscan penetrate into siRNA strands due to the establishment of hydrophobic interactions. Finally, in the par-ticular case of first generation dendrimer [G1O3(NMe2(CH2)2OH))6]6+; the presence of hydroxyl groupsreinforces dendriplex stability by hydrogen bonds formation. However, since these small dendrimers donot cover the RNA, only higher generation derivatives protect RNA from degradation.

Název v anglickém jazyce

Study of non-covalent interactions on dendriplex formation: Influence of hydrophobic, electrostatic and hydrogen bonds interactions

Popis výsledku anglicky

tThe interaction of a double stranded small interference RNA (siRNA Nef) with cationic carbosilane den-drimers of generations 1-3 with two different ammonium functions at the periphery ([MINUS SIGN NMe2R]+, R = Me,(CH2)2OH) has been studied by experimental techniques (zeta potential, electrophoresis, single moleculepulling experiments) and molecular dynamic calculations. These studies state the presence of differentforces on dendriplex formation, depending on generation and type of ammonium group. Whilst for higherdendrimers electrostatic forces mainly drive the stability of dendriplexes, first generation compoundscan penetrate into siRNA strands due to the establishment of hydrophobic interactions. Finally, in the par-ticular case of first generation dendrimer [G1O3(NMe2(CH2)2OH))6]6+; the presence of hydroxyl groupsreinforces dendriplex stability by hydrogen bonds formation. However, since these small dendrimers donot cover the RNA, only higher generation derivatives protect RNA from degradation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Svazek periodika

2018

Číslo periodika v rámci svazku

162

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

380-388

Kód UT WoS článku

000425076500044

EID výsledku v databázi Scopus

2-s2.0-85037543540