A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F18%3A43894071" target="_blank" >RIV/44555601:13440/18:43894071 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/18:00490109 RIV/60461373:22310/18:43917024

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0927776518300560?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0927776518300560?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2018.01.048" target="_blank" >10.1016/j.colsurfb.2018.01.048</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils

Popis výsledku v původním jazyce

tHerein, we report a novel concept of low-cost flexible platform for fluorescence-based biosensor. Thesurface of polyethylene naphthalate (PEN) foil was exposed to KrF excimer laser through a photolito-graphic contact mask. Laser initiated surface modification resulted in micro-patterned areas with surfacefunctional groups available for localized covalent immobilization of biotin. High affinity binding protein(albumin-binding domain (ABD) of protein G, Streptococcus G148) recognizing human serum albumin(HSA), genetically fused with streptavidin (SA-ABDwt), was immobilized on the micro-patterned surfacethrough biotin-streptavidin coupling. Fluorescently labelled HSA analyte was detected in several block-ing environments, in 1% bovine serum albumin (BSA) and 6% fetal serum albumin (FBS), respectively. Weconclude that the presented novel concept enabled us to micropattern functional biosensing layers onthe surface of PEN foil in a fast and easy way. It brings all necessary aspects for continuous roll-to-rollfabrication of low-cost optical bioanalytical devices.

Název v anglickém jazyce

A simple approach for fabrication of optical affinity-based bioanalytical microsystem on polymeric PEN foils

Popis výsledku anglicky

tHerein, we report a novel concept of low-cost flexible platform for fluorescence-based biosensor. Thesurface of polyethylene naphthalate (PEN) foil was exposed to KrF excimer laser through a photolito-graphic contact mask. Laser initiated surface modification resulted in micro-patterned areas with surfacefunctional groups available for localized covalent immobilization of biotin. High affinity binding protein(albumin-binding domain (ABD) of protein G, Streptococcus G148) recognizing human serum albumin(HSA), genetically fused with streptavidin (SA-ABDwt), was immobilized on the micro-patterned surfacethrough biotin-streptavidin coupling. Fluorescently labelled HSA analyte was detected in several block-ing environments, in 1% bovine serum albumin (BSA) and 6% fetal serum albumin (FBS), respectively. Weconclude that the presented novel concept enabled us to micropattern functional biosensing layers onthe surface of PEN foil in a fast and easy way. It brings all necessary aspects for continuous roll-to-rollfabrication of low-cost optical bioanalytical devices.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20501 - Materials engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Svazek periodika

2018

Číslo periodika v rámci svazku

165

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

28-36

Kód UT WoS článku

000432506000004

EID výsledku v databázi Scopus

2-s2.0-85041839569