Direct immobilization of biotin on the micro-patterned PEN foil treated by excimer laser

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22310%2F15%3A43900214" target="_blank" >RIV/60461373:22310/15:43900214 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/44555601:13440/15:43886684

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0927776515001083" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0927776515001083</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2015.02.032" target="_blank" >10.1016/j.colsurfb.2015.02.032</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Direct immobilization of biotin on the micro-patterned PEN foil treated by excimer laser

Popis výsledku v původním jazyce

Polymers with functionalized surfaces have attracted a lot of attention in the last few years. Due to the progress in the techniques of polymer micro-patterning, miniaturized bioanalytical assays and bio-compatible devices can be developed. In the presented work, we performed surface modification of polyethylene naphthalate (PEN) foil by an excimer laser beam through a photolithographic contact mask. The aim was to fabricate micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. It was found out that depending on the properties of the laser scans, a polymer surface exhibits different degrees of modification and as a consequence, different degrees of surface biotinylation can be achieved. Severalaffinity tests with optical detection of fluorescently labeled streptavidin were successfully performed on biotinylated micro-patterns of a PEN foil. The polymer surface properties were also evaluated by electrokinetic analysis, Fourier t

Název v anglickém jazyce

Direct immobilization of biotin on the micro-patterned PEN foil treated by excimer laser

Popis výsledku anglicky

Polymers with functionalized surfaces have attracted a lot of attention in the last few years. Due to the progress in the techniques of polymer micro-patterning, miniaturized bioanalytical assays and bio-compatible devices can be developed. In the presented work, we performed surface modification of polyethylene naphthalate (PEN) foil by an excimer laser beam through a photolithographic contact mask. The aim was to fabricate micro-patterned areas with surface functional groups available for localized covalent immobilization of biotin. It was found out that depending on the properties of the laser scans, a polymer surface exhibits different degrees of modification and as a consequence, different degrees of surface biotinylation can be achieved. Severalaffinity tests with optical detection of fluorescently labeled streptavidin were successfully performed on biotinylated micro-patterns of a PEN foil. The polymer surface properties were also evaluated by electrokinetic analysis, Fourier t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JJ - Ostatní materiály

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B: Biointerfaces

ISSN

0927-7765

e-ISSN

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Svazek periodika

128

Číslo periodika v rámci svazku

APR 1 2015

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

363-369

Kód UT WoS článku

000353930000046

EID výsledku v databázi Scopus

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