Immunocapturing rare cells from blood: A simple and robust microsystem approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F44555601%3A13440%2F23%3A43897652" target="_blank" >RIV/44555601:13440/23:43897652 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0956566323000970?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0956566323000970?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bios.2023.115155" target="_blank" >10.1016/j.bios.2023.115155</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunocapturing rare cells from blood: A simple and robust microsystem approach

Popis výsledku v původním jazyce

Cell immunocapture microsystems are a fast-emerging field with several potential medical diagnostic applications.Isolation and quantification of circulating rare cells (CRCs) show great importance in the early stages ofdisease diagnostics and prognostics. Here, we present a simple and robust stop-flow microsystem (fabricated by acombination of glass microblasting and 3D printing) based on a planar antibody-coated surface that is effective inthe immunocapture of the model as well as naturally occurring rare cells. A chip with a planar immunocapturechannel working in the so-called stop-flow dynamic regime was designed to enable monitoring the efficiency ofthe cell capture by fluorescence microscopy. Up to 90% immunocapture efficiency of MCF-7 cells spiked intowhole blood on CD326 antibody-coated planar surfaces was achieved. We discuss the role of the planar surfacemodifications, the influence of the set stop-flow dynamic conditions, and medium complexity on the efficiency ofcell immunocapture. The presented results could be further employed in the design of microsystems for cell-sizeindependentisolation and identification of rare cells from blood.

Název v anglickém jazyce

Immunocapturing rare cells from blood: A simple and robust microsystem approach

Popis výsledku anglicky

Cell immunocapture microsystems are a fast-emerging field with several potential medical diagnostic applications.Isolation and quantification of circulating rare cells (CRCs) show great importance in the early stages ofdisease diagnostics and prognostics. Here, we present a simple and robust stop-flow microsystem (fabricated by acombination of glass microblasting and 3D printing) based on a planar antibody-coated surface that is effective inthe immunocapture of the model as well as naturally occurring rare cells. A chip with a planar immunocapturechannel working in the so-called stop-flow dynamic regime was designed to enable monitoring the efficiency ofthe cell capture by fluorescence microscopy. Up to 90% immunocapture efficiency of MCF-7 cells spiked intowhole blood on CD326 antibody-coated planar surfaces was achieved. We discuss the role of the planar surfacemodifications, the influence of the set stop-flow dynamic conditions, and medium complexity on the efficiency ofcell immunocapture. The presented results could be further employed in the design of microsystems for cell-sizeindependentisolation and identification of rare cells from blood.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biosensors and Bioelectronics

ISSN

0956-5663

e-ISSN

1873-4235

Svazek periodika

2023

Číslo periodika v rámci svazku

227

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

"nestrankovano"

Kód UT WoS článku

000946548100001

EID výsledku v databázi Scopus

2-s2.0-85148544444