Modified biosensor for cholinesterase inhibitors with Guinea Green B as the colour indicator

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F45808121%3A_____%2F18%3AN0000006" target="_blank" >RIV/45808121:_____/18:N0000006 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Modified biosensor for cholinesterase inhibitors with Guinea Green B as the colour indicator

Popis výsledku v původním jazyce

Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable and very simple detection of agents in air, in water and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualised by using Ellman’s reagent as a chromogenic indicator. The modification basically consists of a much more distinct colour response of the biosensor, attained through optimisation of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 seconds, inhibition efficiency 25%).

Název v anglickém jazyce

Modified biosensor for cholinesterase inhibitors with Guinea Green B as the colour indicator

Popis výsledku anglicky

Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable and very simple detection of agents in air, in water and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualised by using Ellman’s reagent as a chromogenic indicator. The modification basically consists of a much more distinct colour response of the biosensor, attained through optimisation of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 seconds, inhibition efficiency 25%).

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/VI20152018024" target="_blank" >VI20152018024: NOVÉ TECHNOLOGIE PRO OSOBNÍ DETEKTOR INHIBITORŮ CHOLINESTERÁZ</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biosensors2018

ISSN

2079-6374

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

81-90

Kód UT WoS článku

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EID výsledku v databázi Scopus

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