Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22320%2F18%3A43916239" target="_blank" >RIV/60461373:22320/18:43916239 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/18:00324294 RIV/70565813:_____/18:N0000004

Výsledek na webu

<a href="https://www.mdpi.com/2079-6374/8/3/81" target="_blank" >https://www.mdpi.com/2079-6374/8/3/81</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/bios8030081" target="_blank" >10.3390/bios8030081</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator

Popis výsledku v původním jazyce

Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable, and very simple detection of agents in air, in water, and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualized by using Ellman&apos;s reagent as a chromogenic indicator. The modification basically consists of a much more distinct color response of the biosensor, attained through optimization of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin, and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 s, inhibition efficiency 25%).

Název v anglickém jazyce

Modified Biosensor for Cholinesterase Inhibitors with Guinea Green B as the Color Indicator

Popis výsledku anglicky

Colorimetric biosensors of cholinesterase inhibitors are ideal for fast, reliable, and very simple detection of agents in air, in water, and on surfaces. This paper describes an innovation of the Czech Detehit biosensor, which is based on a biochemical enzymatic reaction visualized by using Ellman&apos;s reagent as a chromogenic indicator. The modification basically consists of a much more distinct color response of the biosensor, attained through optimization of the reaction system by using Guinea Green B as the indicator. The performance of the modified biosensor was verified on the chemical warfare agents (sarin, soman, cyclosarin, and VX) in water. The detection limits ascertained visually (with the naked eye) were about 0.001 µg/mL in water (exposure time 60 s, inhibition efficiency 25%).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

<a href="/cs/project/VI20152018024" target="_blank" >VI20152018024: NOVÉ TECHNOLOGIE PRO OSOBNÍ DETEKTOR INHIBITORŮ CHOLINESTERÁZ</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biosensors

ISSN

2079-6374

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

10

Strana od-do

—

Kód UT WoS článku

000448396500025

EID výsledku v databázi Scopus

2-s2.0-85054766054