Combined silk fibroin/nanohydroxyapatite scaffold with antibiotic addition for bone regeneration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F19%3A00008529" target="_blank" >RIV/46747885:24210/19:00008529 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24620/19:00008529

Výsledek na webu

<a href="https://www.setcor.org/conferences/sms-2019" target="_blank" >https://www.setcor.org/conferences/sms-2019</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Combined silk fibroin/nanohydroxyapatite scaffold with antibiotic addition for bone regeneration

Popis výsledku v původním jazyce

Osteomyelitis has still been a challenging sur-gical issue in current orthopaedy. Approximate-ly 80% of all chronic osteomyelitis cases are caused by posttraumatic or postoperative in-flammation. About one third of these cases develops into chronic state because of a for-mation of bacterial biofilm on the implanted materials. A biocompatible material with anti-bacterial activity, acceptable morphology and degradation rate would be an optimal choice for bone replacement. Following the biomimetic approach we created a scaffold combining organic and inorganic components, and we enhanced it with broad-spectrum antibiotic in order to prevent postop-erative bacterial contamination. The organic part of the scaffold is made of silk fibroin, which is a natural fibrous protein derived from Bombyx mori cocoons. Silk fibroin has been currently intensely studied for an application in tissue engineering and regenerative medicine due to its very good compatibility on human cell lines. The inorganic part of the scaffolds represents hydroxyapatite, which is the main mineral component of human bones. Its con-tent naturally varies from 50 to 70%. We pre-pared a set of scaffolds with increasing nano-hydroxyapatite content up to 50% (w/w). The influence of nanohydroxyapatite content along with addition of antibiotic on bone cell line viability and metabolic activity was tested. We also studied morphology of pristine scaffolds and antimicrobial activity towards different bacterial strains. Both hydroxyapatite and anti-biotic content did not cause cytotoxic effect on selected human cell lines when tested under in vitro conditions. Addition of the antibiotic markedly affected bacterial growth both inside the scaffold and in its close surrounding with no effect on the scaffold’s mechanical stability or morphology of all samples with hydroxyap-atite content. The silk fibro-in/nanohydroxyapatite scaffold has a strong potencial to be a suitable scaffold for bone tissue. (Poster presentation)

Název v anglickém jazyce

Combined silk fibroin/nanohydroxyapatite scaffold with antibiotic addition for bone regeneration

Popis výsledku anglicky

Osteomyelitis has still been a challenging sur-gical issue in current orthopaedy. Approximate-ly 80% of all chronic osteomyelitis cases are caused by posttraumatic or postoperative in-flammation. About one third of these cases develops into chronic state because of a for-mation of bacterial biofilm on the implanted materials. A biocompatible material with anti-bacterial activity, acceptable morphology and degradation rate would be an optimal choice for bone replacement. Following the biomimetic approach we created a scaffold combining organic and inorganic components, and we enhanced it with broad-spectrum antibiotic in order to prevent postop-erative bacterial contamination. The organic part of the scaffold is made of silk fibroin, which is a natural fibrous protein derived from Bombyx mori cocoons. Silk fibroin has been currently intensely studied for an application in tissue engineering and regenerative medicine due to its very good compatibility on human cell lines. The inorganic part of the scaffolds represents hydroxyapatite, which is the main mineral component of human bones. Its con-tent naturally varies from 50 to 70%. We pre-pared a set of scaffolds with increasing nano-hydroxyapatite content up to 50% (w/w). The influence of nanohydroxyapatite content along with addition of antibiotic on bone cell line viability and metabolic activity was tested. We also studied morphology of pristine scaffolds and antimicrobial activity towards different bacterial strains. Both hydroxyapatite and anti-biotic content did not cause cytotoxic effect on selected human cell lines when tested under in vitro conditions. Addition of the antibiotic markedly affected bacterial growth both inside the scaffold and in its close surrounding with no effect on the scaffold’s mechanical stability or morphology of all samples with hydroxyap-atite content. The silk fibro-in/nanohydroxyapatite scaffold has a strong potencial to be a suitable scaffold for bone tissue. (Poster presentation)

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.