Study of the interaction of mouse fibroblasts with biodegradable polyesters micro/nanofibrous materials using by live cell imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F22%3A00010496" target="_blank" >RIV/46747885:24210/22:00010496 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24510/22:00010496

Výsledek na webu

<a href="http://dx.doi.org/10.37904/nanocon.2022.4607" target="_blank" >http://dx.doi.org/10.37904/nanocon.2022.4607</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2022.4607" target="_blank" >10.37904/nanocon.2022.4607</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of the interaction of mouse fibroblasts with biodegradable polyesters micro/nanofibrous materials using by live cell imaging

Popis výsledku v původním jazyce

Tissue engineering and regenerative medicine are ever-growing multidisciplinary fields. Along with the development of tissue carriers, methods for testing the biocompatibility of these materials are also being developed. This work deals with research, development, and optimisation of the preparation of a fibre system intended for monitoring the direct interaction of the 3T3 mouse fibroblast cell line with polymeric materials in real-time (live cell imaging). The fabricated microfibre model (grid) was seeded with the 3T3 mouse fibroblast cell line, and the interaction between the materials and the cells was monitored for three days by optical microscope. The interaction between the materials and the cells was observed during a picture of cells on the fibres. The advantage of this method is the absence of other chemicals like chemicals for the visualisation of cells (fluorescence staining) or chemicals for measuring cell viability because cells adhering to fibres are visible in transmitted light.

Název v anglickém jazyce

Study of the interaction of mouse fibroblasts with biodegradable polyesters micro/nanofibrous materials using by live cell imaging

Popis výsledku anglicky

Tissue engineering and regenerative medicine are ever-growing multidisciplinary fields. Along with the development of tissue carriers, methods for testing the biocompatibility of these materials are also being developed. This work deals with research, development, and optimisation of the preparation of a fibre system intended for monitoring the direct interaction of the 3T3 mouse fibroblast cell line with polymeric materials in real-time (live cell imaging). The fabricated microfibre model (grid) was seeded with the 3T3 mouse fibroblast cell line, and the interaction between the materials and the cells was monitored for three days by optical microscope. The interaction between the materials and the cells was observed during a picture of cells on the fibres. The advantage of this method is the absence of other chemicals like chemicals for the visualisation of cells (fluorescence staining) or chemicals for measuring cell viability because cells adhering to fibres are visible in transmitted light.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

20500 - Materials engineering

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON Conference Proceedings - International Conference on Nanomaterials

ISBN

—

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

480

Strana od-do

188-193

Název nakladatele

Tanger Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

1. 1. 2022

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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