Finite element analysis of microfluidic device for Escherichia coli separation from blood-cells for early diagnosis of sepsis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24210%2F25%3A00013740" target="_blank" >RIV/46747885:24210/25:00013740 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24620/25:00013740

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2590123025026271?pes=vor&utm_source=scopus&getft_integrator=scopus" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2590123025026271?pes=vor&utm_source=scopus&getft_integrator=scopus</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rineng.2025.106558" target="_blank" >10.1016/j.rineng.2025.106558</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Finite element analysis of microfluidic device for Escherichia coli separation from blood-cells for early diagnosis of sepsis

Popis výsledku v původním jazyce

Blood cell separation and infectious microorganism detection were investigated using COMSOL Multiphysics (R) 5.3a simulation software for finite element analysis (FEA). FEA was used as a preliminary study to optimize physical prototypes and experiments using related numerical techniques. The separation of white blood cells (WBCs) from other particles in the blood sample was achieved through solid-phase microextraction, which relies on size-based separation, whereas the detection of infectious microorganisms was based on their electrical properties. Both these separation and detection methods can be implemented on a microfluidic device with simple processes. The FEA studies demonstrate an efficient microfluidic channel design for optimized flow, enabling blood cell separation through microchannel width manipulation and the detection of infectious microorganisms via applied dielectrophoretic force. The simulation results indicated that a flow rate of 0.05 mL/ min at 3 MHz yields a high recovery efficiency of 85 %, with separation accomplished in below 10 min. Furthermore, fluorescence-based cell counting confirmed a WBC count of 2.0 x 109/L in the designated counting channel, aligning well with the simulation results. The findings proved that early diagnosis of sepsis is effectively possible through rapid WBC analysis and identification of specific infectious microorganisms using a microfluidic device, enabling timely point-of-care treatment without side effects.

Název v anglickém jazyce

Finite element analysis of microfluidic device for Escherichia coli separation from blood-cells for early diagnosis of sepsis

Popis výsledku anglicky

Blood cell separation and infectious microorganism detection were investigated using COMSOL Multiphysics (R) 5.3a simulation software for finite element analysis (FEA). FEA was used as a preliminary study to optimize physical prototypes and experiments using related numerical techniques. The separation of white blood cells (WBCs) from other particles in the blood sample was achieved through solid-phase microextraction, which relies on size-based separation, whereas the detection of infectious microorganisms was based on their electrical properties. Both these separation and detection methods can be implemented on a microfluidic device with simple processes. The FEA studies demonstrate an efficient microfluidic channel design for optimized flow, enabling blood cell separation through microchannel width manipulation and the detection of infectious microorganisms via applied dielectrophoretic force. The simulation results indicated that a flow rate of 0.05 mL/ min at 3 MHz yields a high recovery efficiency of 85 %, with separation accomplished in below 10 min. Furthermore, fluorescence-based cell counting confirmed a WBC count of 2.0 x 109/L in the designated counting channel, aligning well with the simulation results. The findings proved that early diagnosis of sepsis is effectively possible through rapid WBC analysis and identification of specific infectious microorganisms using a microfluidic device, enabling timely point-of-care treatment without side effects.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21100 - Other engineering and technologies

Projekt

<a href="/cs/project/EF16_025%2F0007293" target="_blank" >EF16_025/0007293: Modulární platforma pro autonomní podvozky specializovaných elektrovozidel pro dopravu nákladu a zařízení</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Results in Engineering>

ISSN

2590-1230

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

SEP

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

001544957000001

EID výsledku v databázi Scopus

2-s2.0-105012267348