Electrospun silica nanofibres as multifunctional substrate for drug delivery and tissue regeneration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24220%2F23%3A00012229" target="_blank" >RIV/46747885:24220/23:00012229 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/46747885:24510/23:00012229 RIV/46747885:24620/23:00012229

Výsledek na webu

<a href="https://www.liebertpub.com/doi/10.1089/ten.tea.2023.29046.abstracts" target="_blank" >https://www.liebertpub.com/doi/10.1089/ten.tea.2023.29046.abstracts</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrospun silica nanofibres as multifunctional substrate for drug delivery and tissue regeneration

Popis výsledku v původním jazyce

Silica nanofibres combine traditional properties of nanofibres, based on their structure, and advantages of inorganic bioactive materials. The aim of this paper is to outline properties and performance of silica nanofibres as biocompatible, biodegradable, and easy to modify high performance material for regenerative medicine and drug delivery. Methodology: Silica nanofibres were prepared by sol-gel method and needle-less electrospinning, which led to formation of nanofibrous matrix of 5 – 30 g/m2 and mean fibre diameter 180 – 850 nm depending on the spinning conditions. Biocompatibility was tested in vitro on several model cell lines including 3T3-A31 fibroblasts, Hacat keratinocytes, Vero cells and HepG2 hepatocyte-like cells in compliance to ISO 10993-5. Biodegradation of silica nanofibres was evaluated in vitro under simulated conditions (37 °C, SBF). Silica release into the SBF was measured using ICP-MS. Impact of degradation on the surface morphology was evaluated by electron microscopy (SEM). Surface availability for functionalization and its impact on relevant properties was tested by APTES aminosilane silanization. Results: Silica nanofibres, obtained by electrospinning, were confirmed to promote multitissue biocompatibility in vitro. Fast degradation under simulated conditions in vitro was observed with surface erosion appearing after 24 hours in simulated body fluid (SBF) with limited swelling and sustained integrity of the nanofibrous matrix. Silica released upon degradation in form of orthosilicic acid, was confirmed to have a beneficial impact on cellular proliferation in vitro, which is known effect provided silica nanomaterials in general. Successful grafting of –NH2 amino group by silanization of surface silanol group was confirmed without relevant impact on the fibre morphology or integrity. Positive impact of the silanization process on biocompatibility was verified.

Název v anglickém jazyce

Electrospun silica nanofibres as multifunctional substrate for drug delivery and tissue regeneration

Popis výsledku anglicky

Silica nanofibres combine traditional properties of nanofibres, based on their structure, and advantages of inorganic bioactive materials. The aim of this paper is to outline properties and performance of silica nanofibres as biocompatible, biodegradable, and easy to modify high performance material for regenerative medicine and drug delivery. Methodology: Silica nanofibres were prepared by sol-gel method and needle-less electrospinning, which led to formation of nanofibrous matrix of 5 – 30 g/m2 and mean fibre diameter 180 – 850 nm depending on the spinning conditions. Biocompatibility was tested in vitro on several model cell lines including 3T3-A31 fibroblasts, Hacat keratinocytes, Vero cells and HepG2 hepatocyte-like cells in compliance to ISO 10993-5. Biodegradation of silica nanofibres was evaluated in vitro under simulated conditions (37 °C, SBF). Silica release into the SBF was measured using ICP-MS. Impact of degradation on the surface morphology was evaluated by electron microscopy (SEM). Surface availability for functionalization and its impact on relevant properties was tested by APTES aminosilane silanization. Results: Silica nanofibres, obtained by electrospinning, were confirmed to promote multitissue biocompatibility in vitro. Fast degradation under simulated conditions in vitro was observed with surface erosion appearing after 24 hours in simulated body fluid (SBF) with limited swelling and sustained integrity of the nanofibrous matrix. Silica released upon degradation in form of orthosilicic acid, was confirmed to have a beneficial impact on cellular proliferation in vitro, which is known effect provided silica nanomaterials in general. Successful grafting of –NH2 amino group by silanization of surface silanol group was confirmed without relevant impact on the fibre morphology or integrity. Positive impact of the silanization process on biocompatibility was verified.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

<a href="/cs/project/EF16_019%2F0000843" target="_blank" >EF16_019/0000843: Hybridní materiály pro hierarchické struktury</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů