Platelet interaction with nano- and microfibrous materials for the tissue engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24410%2F16%3A00003640" target="_blank" >RIV/46747885:24410/16:00003640 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/27283933:_____/17:00005364 RIV/46747885:24410/17:00005364

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Platelet interaction with nano- and microfibrous materials for the tissue engineering

Popis výsledku v původním jazyce

Tissue engineering (TE) is an interdisciplinary field that focuse on the development of biological substitutes that restore, maintain or improve tissue/organ functions. Nano/microfibrous materials are traditionally used in TE since their morphology resembles significant features of native extracellular matrix (ECM). Fibrous scaffolds have a high surface to volume ratio which is thought to promote cell adhesion, migration, proliferation and differentiation. As polymeric fibrous scaffold fabrication techniques strive to create structures that mimics the structure and function of ECM, the need for increased scaffold bioactivity becomes more pronounced. Platelet-rich plasma (PRP) contain over 300 bioactive molecules. PRP has the potential to deliver a combination of GF and cytokines capable of stimulating cellular activity. Incorporation of platelets into scaffold will help to promote scaffold integration within native tissues and increase the overall patency of fibrous structure. Aim of the study: We used PRP to study interaction between native platelets and fibrous materials with the different morphologies (porosity, fibres diameter – micro, nano). Materials and methods: PRP was obtained from buffy coats (7x106 PLT/ml). Fibrous materials were made from polycaprolactone by electrospinning, centrifugal spinning and met-blown methods. Prepared and sterilized materials were incubated for 2h with PRP. The degree of platelet adhesion was measured using MTT method. Interaction of PLT with materials was evaluated by fluorescence microscopy (FITC-anti CD41) and electrone microscopy (SEM). The GF release was monitored by SDS PAGE.

Název v anglickém jazyce

Platelet interaction with nano- and microfibrous materials for the tissue engineering

Popis výsledku anglicky

Tissue engineering (TE) is an interdisciplinary field that focuse on the development of biological substitutes that restore, maintain or improve tissue/organ functions. Nano/microfibrous materials are traditionally used in TE since their morphology resembles significant features of native extracellular matrix (ECM). Fibrous scaffolds have a high surface to volume ratio which is thought to promote cell adhesion, migration, proliferation and differentiation. As polymeric fibrous scaffold fabrication techniques strive to create structures that mimics the structure and function of ECM, the need for increased scaffold bioactivity becomes more pronounced. Platelet-rich plasma (PRP) contain over 300 bioactive molecules. PRP has the potential to deliver a combination of GF and cytokines capable of stimulating cellular activity. Incorporation of platelets into scaffold will help to promote scaffold integration within native tissues and increase the overall patency of fibrous structure. Aim of the study: We used PRP to study interaction between native platelets and fibrous materials with the different morphologies (porosity, fibres diameter – micro, nano). Materials and methods: PRP was obtained from buffy coats (7x106 PLT/ml). Fibrous materials were made from polycaprolactone by electrospinning, centrifugal spinning and met-blown methods. Prepared and sterilized materials were incubated for 2h with PRP. The degree of platelet adhesion was measured using MTT method. Interaction of PLT with materials was evaluated by fluorescence microscopy (FITC-anti CD41) and electrone microscopy (SEM). The GF release was monitored by SDS PAGE.

Druh

O - Ostatní výsledky

CEP obor

JI - Kompositní materiály

OECD FORD obor

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Projekt

<a href="/cs/project/NV15-29241A" target="_blank" >NV15-29241A: Nanovlákenná biodegradabilní maloprůměrová cévní náhrada</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů