Nanofibre Biomass Carriers as a Valuable Tool for Analysis of Microbial Community At Polluted Locality

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F14%3A%230000619" target="_blank" >RIV/46747885:24620/14:#0000619 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.biotech2014.cz" target="_blank" >http://www.biotech2014.cz</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nanofibre Biomass Carriers as a Valuable Tool for Analysis of Microbial Community At Polluted Locality

Popis výsledku v původním jazyce

Chlorinated ethenes (CE) are the second most common soil contaminant worldwide; hence there is long-term experience with a range of clean-up techniques for such pollutants. One of the most successful methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation of CE, stimulated by organic substrate application in situ. At some sites, however, cis1,2-DCE and cancerous vinyl chloride have been shown to accumulate due to a reduction in ERD efficiency, though the mechanisms are not fully understood. A deeper insight into the key-factors affecting the ERD process, therefore, is highly desirable. Here, we demonstrate our initial test of nanofibre biomass carrier composed of sample stabilisation for transport to the laboratory, isolation DNA and PCR amplification . We aim to accelerate, automate and increase the precision of pre-treatment biomass sampling for DNA isolation by replacing expensive and time consuming groundwater filtration by newly developed

Název v anglickém jazyce

Nanofibre Biomass Carriers as a Valuable Tool for Analysis of Microbial Community At Polluted Locality

Popis výsledku anglicky

Chlorinated ethenes (CE) are the second most common soil contaminant worldwide; hence there is long-term experience with a range of clean-up techniques for such pollutants. One of the most successful methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation of CE, stimulated by organic substrate application in situ. At some sites, however, cis1,2-DCE and cancerous vinyl chloride have been shown to accumulate due to a reduction in ERD efficiency, though the mechanisms are not fully understood. A deeper insight into the key-factors affecting the ERD process, therefore, is highly desirable. Here, we demonstrate our initial test of nanofibre biomass carrier composed of sample stabilisation for transport to the laboratory, isolation DNA and PCR amplification . We aim to accelerate, automate and increase the precision of pre-treatment biomass sampling for DNA isolation by replacing expensive and time consuming groundwater filtration by newly developed

Druh

D - Stať ve sborníku

CEP obor

DJ - Znečištění a kontrola vody

OECD FORD obor

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Projekt

<a href="/cs/project/TA02020534" target="_blank" >TA02020534: Integrační technologie pro hodnocení a podporu úplného odstranění chlorovaných etylénů z podzemní vody</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biotech 2014 & 6th Czech - Swiss Symposium with Exhibition

ISBN

978-80-7080-887-0

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

212-213

Název nakladatele

Institute of Chemical Technology

Místo vydání

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Místo konání akce

Praha

Datum konání akce

1. 1. 2014

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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