Nanofibre Biomass Carriers as a Promising Tool for Genetic Analysis of Microbial Community from Highly Polluted Sites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F14%3A%230000620" target="_blank" >RIV/46747885:24620/14:#0000620 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.gsbiconference.elsevier.com/" target="_blank" >http://www.gsbiconference.elsevier.com/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nanofibre Biomass Carriers as a Promising Tool for Genetic Analysis of Microbial Community from Highly Polluted Sites

Popis výsledku v původním jazyce

The applicability of nanofibre material as a carrier for indigenous microbial communities was tested at sites contaminated by chlorinated ethenes (CE). CE are the second most common soil contaminants worldwide. One of the most successful remediation methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation. However, cis1,2-DCE and cancerous vinyl chloride can unpredictably accumulate at some polluted sites during ERD. A deeper insight into the key-factors affecting the ERD process is therefore highly desirable. The nanofibre biomass carriers can help to characterize indigenous microbes in a well-defined and controlled way. The technique includes sample stabilisation for safe transport to a laboratory, isolationDNA and PCR/qPCR amplification. Nanofibre carriers of a high specific surface were installed in polluted underground water for at least two months. Stabilization of biomass on carriers was done four different ways, right after sampling: i

Název v anglickém jazyce

Nanofibre Biomass Carriers as a Promising Tool for Genetic Analysis of Microbial Community from Highly Polluted Sites

Popis výsledku anglicky

The applicability of nanofibre material as a carrier for indigenous microbial communities was tested at sites contaminated by chlorinated ethenes (CE). CE are the second most common soil contaminants worldwide. One of the most successful remediation methods is enhanced reductive dehalogenation (ERD), which utilises anaerobic microbial degradation. However, cis1,2-DCE and cancerous vinyl chloride can unpredictably accumulate at some polluted sites during ERD. A deeper insight into the key-factors affecting the ERD process is therefore highly desirable. The nanofibre biomass carriers can help to characterize indigenous microbes in a well-defined and controlled way. The technique includes sample stabilisation for safe transport to a laboratory, isolationDNA and PCR/qPCR amplification. Nanofibre carriers of a high specific surface were installed in polluted underground water for at least two months. Stabilization of biomass on carriers was done four different ways, right after sampling: i

Druh

O - Ostatní výsledky

CEP obor

DJ - Znečištění a kontrola vody

OECD FORD obor

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Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů