Field study on the dynamics of microbial communities following biostimulation at organochlorine-contaminated sites

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F24%3A00012607" target="_blank" >RIV/46747885:24620/24:00012607 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/24:43930644 RIV/27283933:_____/24:N0000024

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S2213343724026654?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S2213343724026654?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jece.2024.114534" target="_blank" >10.1016/j.jece.2024.114534</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Field study on the dynamics of microbial communities following biostimulation at organochlorine-contaminated sites

Popis výsledku v původním jazyce

Chlorinated ethenes (CEs) have become widespread contaminants in soils, sediments, and groundwater due to extensive historical use, improper handling, and disposal. At many sites, biostimulation is an effective remediation strategy for treating CEs, involving the addition of electron donors to support bacteria capable of degrading these compounds. This study aimed to understand how microbial communities adapt to environmental changes induced by biostimulation interventions. We investigated changes in microbial communities on biomass carriers transferred from a non-biostimulated, CE-contaminated environment to a previously biostimulated environment at two sites. In the non-biostimulated wells, a predominance of bacteria with nitrate-reducing or iron-reducing metabolisms was evident. Some of which are known to aerobically degrade CEs or other organic pollutants. Following the transfer of the carriers, these bacteria were outcompeted by anaerobic microorganisms with respiratory and fermentative metabolisms. The newly formed microbial community on the transferred biomass carriers exhibited greater diversity and higher abundance of organohalide-respiring bacteria. This rapid adaptability indicates the potential for these bacteria to flourish in favorable conditions, particularly at Site 2, where five days after carrier transfer, Dehalococcoides abundance increased 14,200-fold, and the biomarker vcrA increased 28,500-fold. PCoA revealed that that greater differences in hydrogeochemical conditions between biostimulated and non-biostimulated wells resulted in accelerated changes in bacterial community composition and diversity. In conclusion, our study demonstrates a rapid proliferation of CE-degrading bacteria after the transfer of biomass carriers into biostimulated wells at two different sites. The transfer stimulated shifts toward organohalide-respiring and other anaerobic bacteria in biostimulated environment and increased microbial diversity.

Název v anglickém jazyce

Field study on the dynamics of microbial communities following biostimulation at organochlorine-contaminated sites

Popis výsledku anglicky

Chlorinated ethenes (CEs) have become widespread contaminants in soils, sediments, and groundwater due to extensive historical use, improper handling, and disposal. At many sites, biostimulation is an effective remediation strategy for treating CEs, involving the addition of electron donors to support bacteria capable of degrading these compounds. This study aimed to understand how microbial communities adapt to environmental changes induced by biostimulation interventions. We investigated changes in microbial communities on biomass carriers transferred from a non-biostimulated, CE-contaminated environment to a previously biostimulated environment at two sites. In the non-biostimulated wells, a predominance of bacteria with nitrate-reducing or iron-reducing metabolisms was evident. Some of which are known to aerobically degrade CEs or other organic pollutants. Following the transfer of the carriers, these bacteria were outcompeted by anaerobic microorganisms with respiratory and fermentative metabolisms. The newly formed microbial community on the transferred biomass carriers exhibited greater diversity and higher abundance of organohalide-respiring bacteria. This rapid adaptability indicates the potential for these bacteria to flourish in favorable conditions, particularly at Site 2, where five days after carrier transfer, Dehalococcoides abundance increased 14,200-fold, and the biomarker vcrA increased 28,500-fold. PCoA revealed that that greater differences in hydrogeochemical conditions between biostimulated and non-biostimulated wells resulted in accelerated changes in bacterial community composition and diversity. In conclusion, our study demonstrates a rapid proliferation of CE-degrading bacteria after the transfer of biomass carriers into biostimulated wells at two different sites. The transfer stimulated shifts toward organohalide-respiring and other anaerobic bacteria in biostimulated environment and increased microbial diversity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20402 - Chemical process engineering

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

Název periodika

Journal of Environmental Chemical Engineering

ISSN

2213-2929

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

001346527100001

EID výsledku v databázi Scopus

2-s2.0-85207653347