EFFECT OF ELECTRIC CURRENT ON ORGANOHALIDE RESPIRING BACTERIA FROM CONTAMINATED LOCALITY TREATED BY nZVI

Kód výsledku v IS VaVaI

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Nalezeny alternativní kódy

RIV/27283933:_____/18:00005689

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

EFFECT OF ELECTRIC CURRENT ON ORGANOHALIDE RESPIRING BACTERIA FROM CONTAMINATED LOCALITY TREATED BY nZVI

Popis výsledku v původním jazyce

Chlorinated ethenes (CEs) are the second most common contaminant worldwide. Remediation procedures combining treatment with nanoscale zero-valent iron (nZVI) and anaerobic microbial degradation supported by electric current belongs to one of the most successful approaches. Here we describe an effect of electric current on indigenous microbial degraders of CE during a field study. Groundwater samples were taken from anode well, four cathode wells and three control wells and isolated DNA was analysed using real-time PCR. Total bacterial biomass was quantified using 16S rDNA gene. Markers of organohalide respiration included Dehalococcoides sp., Desulfitobacterium spp. and vinyl chloride (VC) reductases vcrA and bvcA. Denitrifying bacteria were determined by nirK marker and sulfate reducing bacteria by apsA2. Representative samples were analysed using NGS method targeted to the V4 region of 16S rDNA gene. qPCR analysis of the anode well reveal significant decrease in all tested markers connected to eminent decrease in pH to strongly acidic values. Cathode wells maintained high level of bacterial growth even if there were slight changes in pH. Composition of bacterial communities in the anode and cathode wells strongly differed and changed over time. In the control wells, a long-term stability in composition of bacterial community was observed. To conclude, electric current caused decrease in organohalide bacteria and other markers in the anode well, all other wells showed ongoing organohalide respiration important for CEs degradation.

Název v anglickém jazyce

EFFECT OF ELECTRIC CURRENT ON ORGANOHALIDE RESPIRING BACTERIA FROM CONTAMINATED LOCALITY TREATED BY nZVI

Popis výsledku anglicky

Chlorinated ethenes (CEs) are the second most common contaminant worldwide. Remediation procedures combining treatment with nanoscale zero-valent iron (nZVI) and anaerobic microbial degradation supported by electric current belongs to one of the most successful approaches. Here we describe an effect of electric current on indigenous microbial degraders of CE during a field study. Groundwater samples were taken from anode well, four cathode wells and three control wells and isolated DNA was analysed using real-time PCR. Total bacterial biomass was quantified using 16S rDNA gene. Markers of organohalide respiration included Dehalococcoides sp., Desulfitobacterium spp. and vinyl chloride (VC) reductases vcrA and bvcA. Denitrifying bacteria were determined by nirK marker and sulfate reducing bacteria by apsA2. Representative samples were analysed using NGS method targeted to the V4 region of 16S rDNA gene. qPCR analysis of the anode well reveal significant decrease in all tested markers connected to eminent decrease in pH to strongly acidic values. Cathode wells maintained high level of bacterial growth even if there were slight changes in pH. Composition of bacterial communities in the anode and cathode wells strongly differed and changed over time. In the control wells, a long-term stability in composition of bacterial community was observed. To conclude, electric current caused decrease in organohalide bacteria and other markers in the anode well, all other wells showed ongoing organohalide respiration important for CEs degradation.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.

Název statě ve sborníku

NANOCON 2017 - Conference Proceedings, 9th International Conference on Nanomaterials - Research and Application

ISBN

9788087294819

ISSN

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e-ISSN

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Počet stran výsledku

7

Strana od-do

390-396

Název nakladatele

TANGER Ltd.

Místo vydání

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Místo konání akce

Brno

Datum konání akce

1. 1. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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