Influence of Hydrogen Peroxide and Sodium Lactate Application on Dehalogenation Bacteria Responsible for Degradation of Chlorinated Ethenes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F16%3A00000924" target="_blank" >RIV/46747885:24620/16:00000924 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4172/1948-5948.C1.026" target="_blank" >http://dx.doi.org/10.4172/1948-5948.C1.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4172/1948-5948.C1.026" target="_blank" >10.4172/1948-5948.C1.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Influence of Hydrogen Peroxide and Sodium Lactate Application on Dehalogenation Bacteria Responsible for Degradation of Chlorinated Ethenes

Popis výsledku v původním jazyce

Due to the extensive using in industry, chlorinated ethenes occurred as common pollutant of environment. As it is environmentally persistent pollutant, its natural biodegradation is very slow. Moreover, natural processes can result in more hazardous by-products as cis-1,2-dichloroethene and vinyl chloride.There are several methods for removing chlorinated ethenes or stimulation natural biodegradation. They are based on injection of chemical agents into remediation wells. Along with natural processes this represents cheap and effective approach. In this study, influence of injection of hydrogen peroxide and sodium lactate on dehalogenation bacteria was examined. Changes were assessed through wide spectrum of molecular genetic markers (16S rRNA gene of dehalogenation bacteria Dehaloccocoides spp., Desulfitobacterium, Dehalobacter and reductive dehalogenase genes (vcrA, bvcA) responsible for dechlorination of vinyl chloride) along with chemical analyses. Application of hydrogen peroxide led to rapid decrease in all markers below detection limit. However, after 13 days, relative abundance of bvcA gene and Desulfitobacterium increased up to 9times, 16times respectively, compared to levels prior to injection. Most values returned within one month after application. Only negligible changes in chlorinated ethenes concentrations were, however, detected, likely due to low dose of hydrogen peroxide.Injection of sodium lactate (substrate enhancing natural biodegradation) resulted in clear increase in marker levels. It indicated positive response of dehalogenation bacteria to biostimulation by sodium lactate. Seven-fold increase in Desulfitobacterium and three-fold increase in Dehaloccocoides spp. accompanied with increase in vcrA gene was detected even after 70 days. Ongoing dehalogenation was also proven by increase in ethene concentration.

Název v anglickém jazyce

Influence of Hydrogen Peroxide and Sodium Lactate Application on Dehalogenation Bacteria Responsible for Degradation of Chlorinated Ethenes

Popis výsledku anglicky

Due to the extensive using in industry, chlorinated ethenes occurred as common pollutant of environment. As it is environmentally persistent pollutant, its natural biodegradation is very slow. Moreover, natural processes can result in more hazardous by-products as cis-1,2-dichloroethene and vinyl chloride.There are several methods for removing chlorinated ethenes or stimulation natural biodegradation. They are based on injection of chemical agents into remediation wells. Along with natural processes this represents cheap and effective approach. In this study, influence of injection of hydrogen peroxide and sodium lactate on dehalogenation bacteria was examined. Changes were assessed through wide spectrum of molecular genetic markers (16S rRNA gene of dehalogenation bacteria Dehaloccocoides spp., Desulfitobacterium, Dehalobacter and reductive dehalogenase genes (vcrA, bvcA) responsible for dechlorination of vinyl chloride) along with chemical analyses. Application of hydrogen peroxide led to rapid decrease in all markers below detection limit. However, after 13 days, relative abundance of bvcA gene and Desulfitobacterium increased up to 9times, 16times respectively, compared to levels prior to injection. Most values returned within one month after application. Only negligible changes in chlorinated ethenes concentrations were, however, detected, likely due to low dose of hydrogen peroxide.Injection of sodium lactate (substrate enhancing natural biodegradation) resulted in clear increase in marker levels. It indicated positive response of dehalogenation bacteria to biostimulation by sodium lactate. Seven-fold increase in Desulfitobacterium and three-fold increase in Dehaloccocoides spp. accompanied with increase in vcrA gene was detected even after 70 days. Ongoing dehalogenation was also proven by increase in ethene concentration.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/GA14-32432S" target="_blank" >GA14-32432S: Mikrobiální meta-omika v souvislosti s fungováním ekosystémů: role populací a jejich metabolických drah v degradaci chlorethenů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů