In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F46747885%3A24620%2F23%3A00012251" target="_blank" >RIV/46747885:24620/23:00012251 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/23:00132394

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S221334372301922X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S221334372301922X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jece.2023.111183" target="_blank" >10.1016/j.jece.2023.111183</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts

Popis výsledku v původním jazyce

The usage of silver nanoparticles (AgNPs) is expected to aggrandize for different ecological applications, owing to inimitable physical, chemical, and biological properties. The need of hour propels the quest for new technologies concerning eco-compatible synthesis of AgNPs and their hybrid forms with higher biocompatibility and maximum efficacy. This study proposes a novel ecofriendly synthesis of antibacterial Poly(N-isopropylacrylamide)-co-sulphonic-Silver (pNSAg) hybrid aided by the aqueous floral extract of Calotropis gigantea. and inquisite its higher mechanistic in vivo biocompatibility with zebrafish. Physiochemical characterization of pNSAg confirmed the hybridization of AgNPs with pNIPAM with thermo-sensitive size variation property as determined by dynamic light scattering, FESEM, and flow cytometry. Comparative antibacterial analysis showed concentration and temperature-dependent higher activity of pNSAg compared to AgNPs at 20 °C. In vivo biocompatibility investigation determined the LC50 of 112.3 µg/mL for pNSAg compared to 51.2 µg/mL for AgNPs with embryonic zebrafish. Mechanistic biocompatibility unraveled it as effect of induction of oxidative stress leading to apoptosis via interaction with metabolic proteins like he1a, Sod1, and p53. The study provided insight into in vivo biocompatibility of polymer-metal nanoparticles hybrid with an eco-compatible approach for their synthesis paving a pathway to ecological and biomedical applications.

Název v anglickém jazyce

In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts

Popis výsledku anglicky

The usage of silver nanoparticles (AgNPs) is expected to aggrandize for different ecological applications, owing to inimitable physical, chemical, and biological properties. The need of hour propels the quest for new technologies concerning eco-compatible synthesis of AgNPs and their hybrid forms with higher biocompatibility and maximum efficacy. This study proposes a novel ecofriendly synthesis of antibacterial Poly(N-isopropylacrylamide)-co-sulphonic-Silver (pNSAg) hybrid aided by the aqueous floral extract of Calotropis gigantea. and inquisite its higher mechanistic in vivo biocompatibility with zebrafish. Physiochemical characterization of pNSAg confirmed the hybridization of AgNPs with pNIPAM with thermo-sensitive size variation property as determined by dynamic light scattering, FESEM, and flow cytometry. Comparative antibacterial analysis showed concentration and temperature-dependent higher activity of pNSAg compared to AgNPs at 20 °C. In vivo biocompatibility investigation determined the LC50 of 112.3 µg/mL for pNSAg compared to 51.2 µg/mL for AgNPs with embryonic zebrafish. Mechanistic biocompatibility unraveled it as effect of induction of oxidative stress leading to apoptosis via interaction with metabolic proteins like he1a, Sod1, and p53. The study provided insight into in vivo biocompatibility of polymer-metal nanoparticles hybrid with an eco-compatible approach for their synthesis paving a pathway to ecological and biomedical applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20701 - Environmental and geological engineering, geotechnics

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of environmental chemical engineering

ISSN

2213-2929

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

—

Kód UT WoS článku

001091697900001

EID výsledku v databázi Scopus

2-s2.0-85173174214