L. ŠVÁBOVÁ - P. SMÝKAL - M. GRIGA - V. ONDŘEJ (2005): Agrobakterální transformace Pisum sativum metodami in vitro a in vivo. Biologia Plantarum, 49, 361-370.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F48392952%3A_____%2F05%3AR0056698" target="_blank" >RIV/48392952:_____/05:R0056698 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Agrobacterium-mediated in vitro and in vivo transformation of Pisum sativum. Biologia Plantarum, 49, 361-370.

Popis výsledku v původním jazyce

Six pea (Pisum sativum L.) cultivars (Adept, Komet, Lantra, Olivín, Oskar, Tyrkys) were transformed via Agrobacterium tumefaciens strain EHA105 with pBIN19 plasmid carrying reporter uidA (ß-glucuronidase, GUS, containing potato ST-LS1 intron) gene underthe CaMV 35S promoter, and selectable marker gene nptII (neomycin phosphotransferase II) under the nos promoter. Two regeneration systems were used: continual shoot proliferation from axillary buds of cotyledonary node in vitro, and in vivo plant regeneration from imbibed germinating seed with removed testa and one cotyledon. The penetration of Agrobacterium into explants during co-cultivation was supported by sonication or vacuum infiltration treatment. The selection of putative transformants in both regeneration systems carried out on media with 100 mg dm-3 kanamycin. The presence of introduced genes was verified histochemically (GUS assay) and by means of PCR and Southern blot analysis in T0 putative transformants and their seed prog

Název v anglickém jazyce

Agrobacterium-mediated in vitro and in vivo transformation of Pisum sativum. Biologia Plantarum, 49, 361-370.

Popis výsledku anglicky

Six pea (Pisum sativum L.) cultivars (Adept, Komet, Lantra, Olivín, Oskar, Tyrkys) were transformed via Agrobacterium tumefaciens strain EHA105 with pBIN19 plasmid carrying reporter uidA (ß-glucuronidase, GUS, containing potato ST-LS1 intron) gene underthe CaMV 35S promoter, and selectable marker gene nptII (neomycin phosphotransferase II) under the nos promoter. Two regeneration systems were used: continual shoot proliferation from axillary buds of cotyledonary node in vitro, and in vivo plant regeneration from imbibed germinating seed with removed testa and one cotyledon. The penetration of Agrobacterium into explants during co-cultivation was supported by sonication or vacuum infiltration treatment. The selection of putative transformants in both regeneration systems carried out on media with 100 mg dm-3 kanamycin. The presence of introduced genes was verified histochemically (GUS assay) and by means of PCR and Southern blot analysis in T0 putative transformants and their seed prog

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

—

Projekt

<a href="/cs/project/QF3072" target="_blank" >QF3072: Tvorba transgenních linií hrachu (Pisum sativum L.) se zvýšenou odolností k virovým patogenům.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologia Plantarum

ISSN

0006-3134

e-ISSN

—

Svazek periodika

—

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

1

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—