The real-time PCR assay for rapid identification of MRSA strains in cow milk

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The real-time PCR assay for rapid identification of MRSA strains in cow milk

Popis výsledku v původním jazyce

The aim of our work was to provide a new, high-throughput and reliable method intended for rapid identification of MRSA in raw cow milk. The duplex real-time PCR assay working with the dual-labelled hydrolysis probes was developed. The sequence specificity of the nuc gene was utilized to design S. aureus specific assay. Detection of the methicillin resistance was based on the mecA gene sequence identifying. A collection of twenty S. aureus/ MRSA reference strains and twenty non - S. aureus strains inclusive Staphylococcus spp. with potential occurrence in milk was analysed to validate the parameters of the method. The incubation of artificially contaminated milk samples in solution of Bird-Parker medium was employed as a selective pre-enrichment step.The PCR assay featured with robust, specific and reproducible amplification. The exclusivity and inclusivity was almost absolute, the limit of the detection was estimated to be tens of copies of S. aureus genome. Our method could be effec

Název v anglickém jazyce

The real-time PCR assay for rapid identification of MRSA strains in cow milk

Popis výsledku anglicky

The aim of our work was to provide a new, high-throughput and reliable method intended for rapid identification of MRSA in raw cow milk. The duplex real-time PCR assay working with the dual-labelled hydrolysis probes was developed. The sequence specificity of the nuc gene was utilized to design S. aureus specific assay. Detection of the methicillin resistance was based on the mecA gene sequence identifying. A collection of twenty S. aureus/ MRSA reference strains and twenty non - S. aureus strains inclusive Staphylococcus spp. with potential occurrence in milk was analysed to validate the parameters of the method. The incubation of artificially contaminated milk samples in solution of Bird-Parker medium was employed as a selective pre-enrichment step.The PCR assay featured with robust, specific and reproducible amplification. The exclusivity and inclusivity was almost absolute, the limit of the detection was estimated to be tens of copies of S. aureus genome. Our method could be effec

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů