A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26784246%3A_____%2F13%3A%230000761" target="_blank" >RIV/26784246:_____/13:#0000761 - isvavai.cz</a>

Výsledek na webu

<a href="http://acta.mendelu.cz/pdf/actaun201361061785.pdf" target="_blank" >http://acta.mendelu.cz/pdf/actaun201361061785.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.11118/actaun201361061785" target="_blank" >10.11118/actaun201361061785</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains

Popis výsledku v původním jazyce

Ther was developed a new more rapid and reliable diagnostic method working on the RT-PCR platform for monitoring of MRSA/S. aureus. PCR of the S. aureus specific nuc gene sequence and the mecA gene sequence was utilised for this purpose. A collection often S. aureus/MRSA reference strains, fifteen genetically related non S. aureus reference strains and fifty-six environmental samples was employed for estimation of the assay performance and parameters. The classic selective cultivation approach with thebiochemical test and agar disk diffusion test was accepted as reference diagnostic method. Our method featured with 100% sensitivity in comparison to reference method. The limit of detection allowed to identify from tens to hundreds copies of S. aureus/MRSA genome. Further, the RT-PCR assay featured with 100% inclusivity and 95% exclusivity at Cq value below 30. We consider our method as ideal for testing of individual suspected colonies, when the results can be acquired in less than 1.

Název v anglickém jazyce

A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains

Popis výsledku anglicky

Ther was developed a new more rapid and reliable diagnostic method working on the RT-PCR platform for monitoring of MRSA/S. aureus. PCR of the S. aureus specific nuc gene sequence and the mecA gene sequence was utilised for this purpose. A collection often S. aureus/MRSA reference strains, fifteen genetically related non S. aureus reference strains and fifty-six environmental samples was employed for estimation of the assay performance and parameters. The classic selective cultivation approach with thebiochemical test and agar disk diffusion test was accepted as reference diagnostic method. Our method featured with 100% sensitivity in comparison to reference method. The limit of detection allowed to identify from tens to hundreds copies of S. aureus/MRSA genome. Further, the RT-PCR assay featured with 100% inclusivity and 95% exclusivity at Cq value below 30. We consider our method as ideal for testing of individual suspected colonies, when the results can be acquired in less than 1.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1210284" target="_blank" >QJ1210284: Zavedení metod detekce MRSA v mase potravinových zvířat a účinných opatření poti jejich šíření v potravinovém řetězci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis

ISSN

1211-8516

e-ISSN

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Svazek periodika

LXI

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

1785-1792

Kód UT WoS článku

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EID výsledku v databázi Scopus

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