Relationship between motility and viability parameters of frozen-thawed bull spermatozoa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F13%3A%230000592" target="_blank" >RIV/49608851:_____/13:#0000592 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.jmbfs.org/issue/special-issue-o-biotechnology-and-quality-of-raw-materials-and-foodstufs-bqrmf/58_jmbs_spalekova-_fbp_b/?issue_id=1716&article_id=57" target="_blank" >http://www.jmbfs.org/issue/special-issue-o-biotechnology-and-quality-of-raw-materials-and-foodstufs-bqrmf/58_jmbs_spalekova-_fbp_b/?issue_id=1716&article_id=57</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Relationship between motility and viability parameters of frozen-thawed bull spermatozoa

Popis výsledku v původním jazyce

The aim of this study was to determine relationship between parameters of spermatozoa motility (total motility - TM and progressive movement - PM) and viability of bull frozen-thawed spermatozoa (dead spermatozoa ratio, apoptotic spermatozoa ratio and plasma membrane integrity). Motility parameters were evaluated using computer-assisted semen analysis (CASA). Parameters of spermatozoa viability were analysed using fluorescent dyes PNA-FITC (plasma membrane), Yo-Pro-1 and propidium iodide (PI). All bulls(n=6) were divided into two groups. First group (n=3) A ? better bulls with total motility after thawing over 40% and the second group (n=3) B ? with total motility lower than 40%. It was observed significantly (P<0.001) higher TM and PM in group A. Nosignificant differences in velocity parameters and ALH between the group A and B were detected. Occurrence of spermatozoa with disrupted membranes, dead/necrotic spermatozoa and apoptotic spermatozoa was significantly lower in the group A

Název v anglickém jazyce

Relationship between motility and viability parameters of frozen-thawed bull spermatozoa

Popis výsledku anglicky

The aim of this study was to determine relationship between parameters of spermatozoa motility (total motility - TM and progressive movement - PM) and viability of bull frozen-thawed spermatozoa (dead spermatozoa ratio, apoptotic spermatozoa ratio and plasma membrane integrity). Motility parameters were evaluated using computer-assisted semen analysis (CASA). Parameters of spermatozoa viability were analysed using fluorescent dyes PNA-FITC (plasma membrane), Yo-Pro-1 and propidium iodide (PI). All bulls(n=6) were divided into two groups. First group (n=3) A ? better bulls with total motility after thawing over 40% and the second group (n=3) B ? with total motility lower than 40%. It was observed significantly (P<0.001) higher TM and PM in group A. Nosignificant differences in velocity parameters and ALH between the group A and B were detected. Occurrence of spermatozoa with disrupted membranes, dead/necrotic spermatozoa and apoptotic spermatozoa was significantly lower in the group A

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/QI91A061" target="_blank" >QI91A061: Vliv rozdílné metabolické aktivity organismu na biologické a oplodňovací charakteristiky gamet dojeného skotu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The journal of microbiology, biotechnology and food sciences

ISSN

1338-5178

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

January 2013 - Special Issue 1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

12

Strana od-do

1580-1591

Kód UT WoS článku

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EID výsledku v databázi Scopus

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