Improvement in Semen Conservation of the Indigenous Czech Endangered Old Kladruber Horse: Special Focus on the Type of Extender and Packaging System

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41110%2F19%3A77779" target="_blank" >RIV/60460709:41110/19:77779 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/19:77779

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0737080618305586?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0737080618305586?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jevs.2018.10.026" target="_blank" >10.1016/j.jevs.2018.10.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Improvement in Semen Conservation of the Indigenous Czech Endangered Old Kladruber Horse: Special Focus on the Type of Extender and Packaging System

Popis výsledku v původním jazyce

The aim of our study was to investigate the effect of two freezing extenders and two packaging systemson motility, plasma membrane integrity, and the apoptotic status of frozen-thawed sper-matozoa of the endangered Old Kladruber stallions. The collected semen was diluted either with Gent or Lactose-EDTA (Lact) extender. Two aliquots of semen fromeach collection diluted in this way were prepared and then loaded into 5-mL aluminum tubes or 0,5-mLplastic straws. After thawing and then at 15 minutes intervals within 1 hour, the samples were analyzedfor motility, PM integrity, and apoptotic changes of the spermatozoa. Using Gent, the samples exhibited higher motility, kinematic parameters, higher representation ofspermatozoa in medium, and fast subpopulation, as well as more spermatozoa with intact PMs and fewerspermatozoa with apoptotic changes compared with Lact extender. Progressive motility and PMintegrity was superior when using Gent and 5-mL aluminum tubes as compared with the rest of thecombination

Název v anglickém jazyce

Improvement in Semen Conservation of the Indigenous Czech Endangered Old Kladruber Horse: Special Focus on the Type of Extender and Packaging System

Popis výsledku anglicky

The aim of our study was to investigate the effect of two freezing extenders and two packaging systemson motility, plasma membrane integrity, and the apoptotic status of frozen-thawed sper-matozoa of the endangered Old Kladruber stallions. The collected semen was diluted either with Gent or Lactose-EDTA (Lact) extender. Two aliquots of semen fromeach collection diluted in this way were prepared and then loaded into 5-mL aluminum tubes or 0,5-mLplastic straws. After thawing and then at 15 minutes intervals within 1 hour, the samples were analyzedfor motility, PM integrity, and apoptotic changes of the spermatozoa. Using Gent, the samples exhibited higher motility, kinematic parameters, higher representation ofspermatozoa in medium, and fast subpopulation, as well as more spermatozoa with intact PMs and fewerspermatozoa with apoptotic changes compared with Lact extender. Progressive motility and PMintegrity was superior when using Gent and 5-mL aluminum tubes as compared with the rest of thecombination

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF EQUINE VETERINARY SCIENCE

ISSN

0737-0806

e-ISSN

1542-7412

Svazek periodika

72

Číslo periodika v rámci svazku

JAN 2019

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

101-107

Kód UT WoS článku

000454122600016

EID výsledku v databázi Scopus

2-s2.0-85056825664